东北虎豹国家公园植物DNA微条形码数据库  被引量:1

A DNA mini-barcode reference library for environmental DNA study in the Northeast Tiger and Leopard National Park

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作  者:吴峰 温佩颖 唐志珍 李青鉴 朱頔 王天明[1] 葛剑平[1] 王红芳[1] WU Feng;WEN Peiying;TANG Zhizhen;LI Qingjian;ZHU Di;WANG Tianming;GE Jianping;WANG Hongfang(Key Laboratory for Biodiversity Science and Ecological Engineering,Ministry of Education,National Forestry and Grassland Administration Key Laboratory for Conservation Ecology in the Northeast Tiger and Leopard National Park,College of Life Sciences,Beijing Normal University,100875,Beijing,China)

机构地区:[1]生物多样性与生态工程教育部重点实验室,东北虎豹国家公园保护生态学国家林草局重点实验室,北京师范大学生命科学学院,北京100875

出  处:《北京师范大学学报(自然科学版)》2023年第4期623-628,共6页Journal of Beijing Normal University(Natural Science)

基  金:国家自然科学基金资助项目(32071494)。

摘  要:DNA宏条形码技术已成为环境DNA分析的常用手段,选择合适的引物并建立本地微条形码数据库,对于环境DNA结果的进一步探究十分重要.本文通过数字模拟PCR技术,对食草动物环境DNA研究中常用的3个微条形码进行比较,发现psbCL具有最高或略低于最高的覆盖度和分辨率,在环境DNA分析中推荐优先使用.利用psbCL建立了东北虎豹国家公园本地微条形码库,同时利用psbCL扩增梅花鹿粪便中的进食物种,以比较本地微条形码库和公共数据库在物种鉴别能力方面的差异.结果表明,本地数据库在属和种水平均提高了鉴别能力.因此,本研究推荐环境DNA分析中建立本地微条形码库,以提高研究的精度,本研究所建立的东北虎豹国家公园植物DNA微条形码数据库也将为未来高精度的环境DNA分析提供基础.DNA metabarcoding has become a powerful tool for analyzing environmental DNA.It is important to select primers and establish local DNA barcode libraries.Three commonly used mini-barcodes for herbivore environmental DNA were compared in this study using in-silico PCR,and psbCL was found to have the highest or slightly lower coverage and resolution,making it the recommended choice for environmental DNA analysis.A local mini-barcode library for Northeast Tiger and Leopard National Park was established using psbCL.The ability to identify species was compared between local mini-barcode library and public databases by amplifying the feeding species in sika deer feces.Data showed that local database improved identification ability at the genus and species levels.This study recommends establishing a local mini-barcode library for environmental DNA analysis to improve accuracy.Plant DNA mini-barcode database for Northeast Tiger and Leopard National Park established in this study will be useful for future high-precision environmental DNA analysis.

关 键 词:植物DNA微条形码 环境DNA DNA宏条形码 条形码数据库 psbCL 

分 类 号:Q943[生物学—植物学]

 

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