猪繁殖与呼吸综合征病毒类NADC30株感染的诊断与防控策略  

Diagnosis,prevention and control strategies of porcine reproductive and respiratory syndrome virus NADC30 like strain infection

作　　者：官土根 Guan Tugen(Fujian Animal Husbaandry Association,Fuzhou 350003)

机构地区：[1]福建省畜牧业协会,福州350003

出　　处：《福建畜牧兽医》2023年第5期47-51,共5页Fujian Journal of Animal Husbandry and Veterinary medicine

摘　　要：2019年10月初,福建闽南地区某猪场出现疑似猪繁殖与呼吸综合征疫情,主要表现为25日龄断乳仔猪出现发热、采食量下降、扎堆、毛长、喘气等现象。为确定发病原因,将随机采集的血清样品165份送至国内某重点实验室进行猪瘟抗体、猪繁殖与呼吸综合征抗体和猪伪狂犬病野毒抗体检测,采集4份肺等病料对猪瘟病毒、猪繁殖与呼吸综合征病毒、伪狂犬病病毒进行PCR检测,PCR产物送上海某公司进行基因测序。结果发现,送检的165份样品中,猪伪狂犬病gE抗体阳性率为0%、gB抗体阳性率为96.19%(101/105),猪瘟抗体阳性率为84.24%(139/165),猪繁殖与呼吸综合征抗体阳性率为92.73%(153/165);病原学检测结果显示,未检出猪瘟病毒、伪狂犬病病毒,但猪繁殖与呼吸综合征病毒的检出率达100%;测序结果表明,4个样品的PCR产物序列均与NADC30毒株的同源性高达92%~93%。结果提示,该场暴发PRRS是类NADC30毒株感染所致,可采取与类NADC30毒株同源性高的疫苗接种和提高生物安全防控水平等方式对该病进行科学防控。Early October 2019,a suspected outbreak of porcine reproductive and respiratory syndrome(PRRS)occurred in a pig farm from southen of Fujian,the main clinlcal symptoms were fever,intake decreased,clustering,hair length,and wheezing in 25 day old weaned piglets.To determine the cause of the disease,165 serum samples were sent to a key laboratory in China for antibodies detection against swine fever,porcine reproductive and respiratory syndrome,and porcine pseudorabies,and 4 samples were detected for swine fever virus,porcine reproductive and respiratory syndrome virus,and porcine pseudorabies virus by PCR.At the same time,the PCR products were sent to a company in Shanghai for sequencing.The results showed that among the 165 samples submitted for testing,the positive rate of antibody against porcine pseudorabies gE is 0%,the positive rate of antibody against porcine pseudorabies gB is 96.19%(101/105),the positive rate of antibodies against swine fever was 84.24%(139/165),and the positive rate of antibodies against PRRS was 92.73%(153/165).The results of pathogenic testing showed that the swine fever virus and pseudorabies virus were negative,but the positive rate of PRRSV were 100%.The results of the homology analysis showed that the sequences were the highest homology(92%~93%)with the NADC30 like strain.The above results suggest that the outbreak of PRRS is caused by the infection of the NADC30 like strain,and the scientific prevention and control measures such as vaccination and improving the level of biosafety prevention and control should be taken to prevent and control the disease.

关 键 词：猪繁殖与呼吸综合征 类NADC30毒株 检测 基因测序 防控 

分 类 号：S858.28[农业科学—临床兽医学]