陕北白绒山羊Notch2基因多态性与绒毛和生长性状的相关性分析  

Polymorphism of Notch 2 Gene and Its Association Analysis withCashmere and Growth Traits in Shaanbei White Cashmere Goats

作　　者：白晶晶 刘晓宇 刘丽娜 宋岩峰 张昕 宋晓越 史雷[1] 李陇平[1] 张磊 朱海鲸[1] 屈雷[1] BAI Jingjing;LIU Xiaoyu;LIU Lina;SONG Yanfeng;ZHANG Xin;SONG Xiaoyue;SHI Lei;LI Longping;ZHANG Lei;ZHU Haijing;QU Lei(Shaanxi Province Engineering&Technology Research Center of Cashmere Goat,Yulin University,Yulin 719000,China;Fugu County Animal Epidemic Prevention and Control Center,Yulin 719000,China)

机构地区：[1]榆林学院,陕西省陕北白绒山羊工程技术研究中心,榆林719000 [2]府谷县动物疫病预防控制中心,榆林719000

出　　处：《中国畜牧兽医》2023年第9期3630-3640,共11页China Animal Husbandry & Veterinary Medicine

基　　金：榆林学院研究生创新基金(2022YLYCX11);国家自然科学基金(32060734);陕西省科技统筹创新工程计划(2014KTDZ02);陕西省教育厅项目重点项目(22JY075);陕西省科技资源开放共享平台(2021PT-017)。

摘　　要：【目的】探究陕北白绒山羊Notch2基因插入/缺失(insertion/deletion, InDel)遗传多态性及其与绒毛和生长性状的相关性,为陕北白绒山羊分子辅助育种提供理论依据。【方法】采集1 469只成年雌性陕北白绒山羊耳组织,提取基因组DNA,并测定绒长、毛长、体长、体高等绒毛与生长性状数据。参考GenBank和Ensembl数据库提供的山羊Notch2基因InDel遗传变异信息设计引物,利用PCR扩增和直接测序法检测Notch2基因17个InDels突变的多态性,并分析其与陕北白绒山羊绒毛和生长性状的相关性。【结果】陕北白绒山羊Notch2基因分别在第15内含子区的12 bp InDel(rs665021370)和第25内含子区的21 bp InDel(rs653705114)存在多态性,且2个InDels位点均存在插入/插入型(II)、杂合型(ID)和缺失/缺失型(DD)3种基因型,这2个位点均属于低度多态(P<0.25),且处于Hardy-Weinberg平衡状态(P>0.05)。关联分析结果表明,Notch2基因rs665021370位点突变与陕北白绒山羊体高或体长呈显著或极显著相关(P<0.05;P<0.01);rs653705114位点突变与陕北白绒山羊毛长、胸宽和体高呈显著或极显著相关(P<0.05;P<0.01)。2个InDels位点不属于强连锁关系。【结论】Notch2基因rs665021370和rs653705114位点突变对陕北白绒山羊绒毛或生长性状有显著影响,可作为陕北白绒山羊优良性状选育的分子标记。【Objective】The purpose of this study was to explore the association between insertion/deletion(InDel)mutation of Notch 2 gene with cashmere and growth traits in Shaanbei White cashmere goats,in order to provide a theoretical basis for molecular breeding of goats.【Method】A total of 1469 adult female Shaanbei White cashmere goats were selected as the research objects,the ear tissue were collected to extract DNA,and their cashmere and growth traits,such as cashmere length,wool length,body length,body height were measured.The primers were designed in reference to the InDel genetic variation information of Notch 2 gene in goats found in GenBank and Ensembl databases,and the 17 InDel mutations of Notch 2 gene were detected by PCR amplification and direct sequencing,and the association between mutation and cashmere and growth traits in Shaanbei White cashmere goats was analyzed.【Result】There were two polymorphism loci in 12 bp InDel(rs665021370)of intron 15 and 21 bp InDel(rs653705114)of intron 25 in Shaanbei White cashmere goats.Three genotypes of insertion/insertion type(II),heterozygous type(ID)and deletion/deletion type(DD)were present at the two InDels,which were all in low polymorphism(P<0.25)and in Hardy-Weinberg equilibrium(P>0.05).The association analysis results showed that rs665021370 mutation of Notch 2 gene was significantly or extremely significantly correlated with body height and body length in Shaanbei White cashmere goats(P<0.05 or P<0.01);rs653705114 mutation was significantly or extremely significantly correlated with wool length,chest width and body height in Shaanbei White cashmere goats(P<0.05 or P<0.01).There was no strong linkage relationship between two InDels.【Conclusion】rs665021370 and rs653705114 mutations of Notch 2 gene had significant effect on cashmere and growth traits in Shaanbei White cashmere goats,which could be used as molecular markers for excellent trait breeding in Shaanbei White cashmere goats.

关 键 词：陕北白绒山羊 Notch2基因 INDEL 多态性 相关性 

分 类 号：S813.3[农业科学—畜牧学]