基于线粒体Cytb基因的凉山黑绵羊群体遗传多样性分析  

Genetic Diversity Analysis of Liangshan Black Sheep PopulationBased on Mitochondrial Cytb Gene

作　　者：乐云峰 黄卫平 刘敏[1] 金素钰[1] 黄林[1] 郑玉才[1] YUE Yunfeng;HUANG Weiping;LIU Min;JIN Suyu;HUANG Lin;ZHENG Yucai(College of Animal&Veterinary Science,Southwest Minzu University,Chengdu 610041,China;Liangshan Yi Autonomous Prefecture Institute of Animal Husbandry and Veterinary Sciences,Xichang 615042,China)

机构地区：[1]西南民族大学畜牧兽医学院,成都610041 [2]凉山彝族自治州畜牧兽医科学研究所,西昌615042

出　　处：《中国畜牧兽医》2023年第9期3671-3679,共9页China Animal Husbandry & Veterinary Medicine

基　　金：西南民族大学研究生创新型科研项目(CX2021095)。

摘　　要：【目的】研究四川省凉山黑绵羊群体的遗传多样性和亲缘关系,以期为当地黑绵羊种质资源保护及合理利用提供科学依据。【方法】利用PCR法扩增凉山黑绵羊线粒体细胞色素b(Cytb)基因并测序,通过BLAST比对确认序列长度和突变位点信息,结合从GenBank中下载的国内外22个绵羊品种的153条绵羊线粒体Cytb基因序列,用Mega 7.0软件进行碱基组成和遗传距离分析,并利用DnaSP 5.0软件进行遗传多样性分析。【结果】凉山黑绵羊群体Cytb基因大小为1 140 bp,未发现插入或缺失,AT含量高于GC含量;采集的60只黑绵羊的Cytb基因共存在13个变异位点,有13种单倍型,其中H2、H3为优势单倍型;凉山黑绵羊单倍型多样性(Hd)、核苷酸多样性(Pi)和平均核苷酸变异数(K)分别为0.674、0.00120和1.371。系统发育树分析结果表明,凉山黑绵羊与乌拉黑羊遗传距离最小,与盘羊遗传距离最大。【结论】凉山州本地黑绵羊Cytb基因遗传多样性较低,其中盐源黑绵羊群体Cytb基因遗传多样性远低于布拖、普格黑绵羊群体,3个黑绵羊群体之间的亲缘关系较近,初步推测具有2个母系起源。凉山州本地黑绵羊群体与乌拉黑羊等亲缘关系最近,与盘羊亲缘关系较远。【Objective】This study was amied to evalute the genetic diversity and genetic relationship of Liangshan black sheep population in Sichuan province,in order to provide scientific basis for the protection and rational utilization of local black sheep germplasm resources.【Method】Mitochondrial cytochrome b(Cytb)gene of Liangshan black sheep was amplified by PCR and sequenced.The sequence length and mutation site information were confirmed by BLAST comparison.Combined with 153 sequences from 22 sheep breeds at home and abroad downloaded from GenBank,mitochondrial Cytb gene sequences of sheep were analyzed using Mega 7.0 software for base composition and genetic distance analysis,and DnaSP 5.0 software was used for genetic diversity analysis.【Result】The Cytb gene size of Liangshan black sheep population was 1140 bp,no insertion or deletion was found,and the AT content was higher than GC content.A total of 13 variation sites were found in the Cytb gene of 60 black sheep,with 13 haplotypes,of which H2 and H3 were the dominant haplotypes.The haplotype diversity(Hd),nucleotide diversity(Pi)and mean nucleotide variance(K)of Liangshan black sheep were 0.674,0.00120 and 1.371,respectively.Phylogenetic tree analysis showed that the genetic distance between Liangshan black sheep and Wula Black sheep was the smallest,and the genetic distance between Liangshan black sheep and Ovis ammon was the largest.【Conclusion】The genetic diversity of Cytb gene in Liangshan local black sheep was low,and the genetic diversity of Cytb gene in Yanyuan black sheep was much lower than that in Butuo and Puge black sheeps.The three black sheep groups were closely related,and it was speculated that they had two matrilineal origins.Liangshan local black sheep had the closest relationship with Wula black sheep,but had a distant relationship with Ovis ammon.

关 键 词：凉山黑绵羊 CYTB基因 遗传多样性 系统进化 

分 类 号：S826.89[农业科学—畜牧学]