LINC01614对非小细胞肺癌A549细胞增殖、侵袭、细胞周期以及凋亡的影响  

Effect of LINC01614 on proliferation,invasion,cell cycle and apoptosis of non-small cell lung cancer A549 cells

作　　者：白钰明 施琳[1,2] 贾永峰 刘霞[1,2] 陈永霞 云芬 BAI Yuming;SHI Lin;JIA Yongfeng;LIU Xia;CHEN Yongxia;YUN Fen(Department of Pathology,the Affiliated Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010000,China;Basic Medical College,Inner Mongolia Medical University,Hohhot,Inner Mongolia 010000,China;Tumor Molecular Diagnostic Laboratory,the Affiliated People’s Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010000,China)

机构地区：[1]内蒙古医科大学附属医院病理科,内蒙古呼和浩特010000 [2]内蒙古医科大学基础医学院,内蒙古呼和浩特010000 [3]内蒙古医科大学附属人民医院肿瘤分子诊断实验室,内蒙古呼和浩特010000

出　　处：《临床肺科杂志》2023年第10期1471-1478,共8页Journal of Clinical Pulmonary Medicine

基　　金：内蒙古自治区自然科学基金项目(No.2018MS08094)。

摘　　要：目的研究LINC01614对非小细胞肺癌A549细胞增殖、侵袭、细胞周期以及凋亡的影响。方法(1)生物信息学分析LINC01614的表达对生存期以及临床病理特征的影响。(2)qRT-PCR法检测LINC01614在A549细胞与人肺上皮细胞BEAS-2B的表达。(3)脂质体转染法向A549细胞中转染LINC01614小干扰RNA(si-LINC01614)。(4)CCK实验与长时间动态活细胞成像仪(IncuCyte S3)检测LINC01614对细胞增殖的影响。(5)Transwell实验检测LINC01614对细胞侵袭的影响。(6)流式细胞术检测LINC01614对细胞周期以及凋亡的影响。结果(1)生物信息学分析显示,与正常组织相比,LINC01614在非小细胞肺癌组织中的表达显著上调(P<0.05);其高表达与远处转移、肿瘤分期相关(P<0.05)。(2)与BEAS-2B细胞相比,LINC01614在A549细胞上的表达显著上调(P<0.05)。(3)沉默LINC01614可抑制A549细胞的增殖、侵袭以及凋亡,使细胞的G0/G1期延长,S期缩短(P<0.05)。结论LINC01614可促进非小细胞肺癌A549细胞的增殖、侵袭以及凋亡。Objective To study the effect of LINC01614 on the proliferation,invasion and apoptosis of non-small cell lung cancer A549 cells.Methods(1)It used bioinformatics to analyze the effect of LINC01614 expression on survival and clinicopathological characteristics.(2)It used qRT-PCR assay to detect the expression of LINC01614 in A549 cells with human lung epithelial cells BEAS-2B.(3)It used lipofection to transfect LINC01614 small interfering RNA(si-LINC01614)into A549 cells.(4)It used CCK assay with prolonged dynamic live cell imager(IncuCyte S3)to detect the effect of LINC01614 on cell proliferation.(5)It used Transwell assay to detect the effect of LINC01614 on cell invasion.(6)It used flow cytometry to detect the effect of LINC01614 on cell cycle as well as apoptosis.Results(1)Bioinformatics analysis showed that the expression of LINC01614 was significantly up-regulated in non-small cell lung cancer tissues while compared with normal tissues(P<0.05).Its high expression was significantly correlated with distant metastasis and tumor stage(P<0.05).(2)The expression of LINC01614 was significantly up-regulated on A549 cells while compared with BEAS-2B cells(P<0.05).(3)Silencing LINC01614 inhibited the proliferation,invasion,and apoptosis of A549 cells,prolonged the G0/G1 phase and shortened the S phase of the cells(P<0.05).Conclusion LINC01614 can promote proliferation,invasion,and apoptosis of non-small cell lung cancer A549 cells.

关 键 词：长链非编码RNA LINC01614 非小细胞肺癌 增殖 侵袭 细胞周期 

分 类 号：R734.2[医药卫生—肿瘤]