23株临床分离单核细胞增生李斯特菌菌株的分子特征  

Study on molecular characterization of 23 clinically isolated Listeria monocytogenes strains

作　　者：廖希玮 高硕[2] 张燕[2] 纪静茹 沈瀚[2] 周万青[2] 吴超[1,3] LIAO Xiwei;GAO Shuo;ZHANG Yan;JI Jingru;SHEN Han;ZHOU Wanqing;WU Chao(Nanjing Drum Tower Hospital,Clinical College of Nanjing Medical University,Nanjing 210008,Jiangsu;Department of Laboratory Medicine,Affiliated Hospital of Medical School,Nanjing University,Nanjing 210008,Jiangsu,China;Department of Infectious diseases,Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University,Nanjing 210008,Jiangsu,China)

机构地区：[1]南京医科大学鼓楼临床医学院,南京210008 [2]南京大学医学院附属鼓楼医院检验科,南京210008 [3]南京大学医学院附属鼓楼医院感染性疾病科,南京210008

出　　处：《临床检验杂志》2023年第7期537-541,共5页Chinese Journal of Clinical Laboratory Science

基　　金：南京大学中国医院改革发展研究院课题;南京鼓楼医院医学发展医疗救助基金会资助项目(NDYG2022048)。

摘　　要：目的分析23株临床分离单核细胞增生李斯特菌菌株的分子特征。方法收集2013年3月至2021年5月南京鼓楼医院临床分离的23株单核细胞增生李斯特菌,通过全基因组测序技术分析菌株毒力基因、耐药基因、谱系、序列分析(ST)、克隆群(CCs);采用多重PCR进行血清分型;采用E-Test试验和纸片扩散法检测菌株药物敏感性。结果23株菌株分属2个谱系,以谱系Ⅱ为主,共12株(52.2%);分为8个ST分型,其中ST8为优势ST型,占39.1%;分为7个CCs(CC87、CC1、CC3、CC5、CC224、CC11和CC8),优势CC型为CC8,共9株(39.1%);所有分离株均携带李斯特菌毒力岛LIPI-1(ActA、hlyA、mpl、plcA、plcB和prfA)和inlA、inlB基因;23株菌株均检出fosX耐药基因,其中1株同时携带tet(M)和dfrG耐药基因;血清群分为1/2a、1/2b及4b,以1/2a为主,共13株(56.5%);所有菌株对青霉素、氨苄西林、美罗培南和万古霉素等9种抗菌药物敏感,仅1株对复方新诺明和四环素耐药菌株。结论临床分离单核细胞增生李斯特菌耐药性较低,并具有遗传多样性,携带多种毒力基因是其致病性重要因素。Objective To analyze the molecular characteristics of 23 clinically isolated Listeria monocytogenes strains.Methods A total of 23 Listeria monocytogenes strains isolated from Nanjing Drum Tower Hospital from March 2013 to May 2021 were collected.The virulence genes,drug resistance genes,genealogy,sequence analysis(ST)and clonal complexes(CCs)of the strains were analyzed by whole genome sequencing technology.Serotyping was carried out by multiplex PCR.Antimicrobial susceptible test were carried out by E-Test and disk diffusion test.Results The 23 strains belonged to 2 lineages,mainly lineageⅡwith 12(52.2%)strains and was divided into 8 ST types,of which ST8 was the dominant type accounting for 39.1%.The genotypes included 7 CC types(CC87,CC1,CC3,CC5,CC224,CC11 and CC8)among which the dominant CC type was CC8 with 9 strains(39.1%).All the isolates carried Listeria virulence islandLIPI-1(ActA,hlyA,mpl,plcA,plcB and prfA)and inlA,inlB genes.The fosX resistance genes were detected in all the 23 strains,of which one strain carried both tet(M)and dfrG resistance genes.The serogroups of the 23 strains were divided into 1/2a,1/2b and 4b,mainly 1/2a,with 13 strains(56.5%).All the strains were susceptible to 9 antimicrobials including penicillin,ampicillin,meropenem and vancomycin,and only 1 strain was resistant to cotrimoxazole and tetracycline.Conclusion The clinically isolated Listeria monocytogenes showed low drug resistance and genetic diversity.A variety of virulence genes carried by these strains should be the important factors in their pathogenicity.

关 键 词：单核细胞增生李斯特菌 全基因组测序 血清型 毒力因子 

分 类 号：R446.5[医药卫生—诊断学]