皮肤光老化动物模型制备要素和受试物数据的文献分析  

Literature Analysis of the Preparation Elements of Animal Models of Skin Photoaging and the Data of Subjects

作　　者：邓亚胜 林江 甘池伶 曾官凤 黄嘉茵 邓慧芳 麻颖贤 韩丝银 DENG Yasheng;LIN Jiang;GAN Chiling;ZENG Guanfeng;HUANG Jiayin;DENG Huifang;MA Yingxian;HAN Siyin(School of Basic Medical Sciences,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学基础医学院,南宁530200

出　　处：《实验动物与比较医学》2023年第4期406-414,共9页Laboratory Animal and Comparative Medicine

基　　金：广西自然科学基金项目“合浦珍珠提取液对中波紫外线诱导人皮肤角质形成细胞光老化干预机制的基础研究”(2020GXNSFBA159039);广西高校中青年教师科研基本能力提升项目“水解南珠粉对人体皮肤美白及抗衰老作用机制的研究”(2019KY0312);广西中医药大学引进博士科研启动基金项目“南珠粉及水解成分的美白活性物质初探和合成筛查研究”(2018BS013);2021年国家级大学生创新创业训练计划项目“南珠粉对Ⅱ级烫伤小鼠皮肤的修复作用研究”(202110600005);2019年广西中医药大学校级科研青年基金项目“南珠提取物对光老化人皮肤细胞的干预作用研究”(2019QN003)。

摘　　要：目的分析皮肤光老化动物模型的造模要素和受试物情况,为该动物模型的制备和完善提供参考,也为科学评价受试物提供依据。方法通过在中国知网、万方、PubMed数据库中检索收集2010—2022年皮肤光老化动物模型制备的相关文献,对文献中记载的模型动物种类、性别、造模方法、造模周期、辐射光源与造模部位距离、累计辐射量、检测指标、受试物(药物或治疗手段)内容进行整理归纳,建立数据库后进行统计分析。结果筛选出257篇符合纳入标准的文献,其中模型动物使用最多的是SKH-1无毛小鼠,其次为SD大鼠和KM小鼠;动物的性别选择以单一雌性为主,常采用中波紫外线(ultraviolet B,UVB)作为辐射光源,辐射光源与造模部位的距离多为30 cm,造模周期多控制在40~60 d;长波紫外线(ultraviolet A,UVA)累计照射剂量在100~150 J/cm2的所占比例最大,中波紫外线(ultraviolet B,UVB)累计照射剂量在5~10 J/cm2的所占比例最大。模型建立后采用的检测指标为皮肤组织病理检查、皮肤组织匀浆、纤维染色、免疫印迹检查等。受试物包括中药、中药提取物、中成药、中药复方、化学药、生物制剂以及其他治疗手段,同时皮肤光老化动物模型还应用于中医外治、物理疗法、阳性对照药方面的临床疗效研究。结论皮肤光老化动物实验常选用SKH-1雌性无毛小鼠,采用UVB作为辐射光源,造模周期多控制在40~60 d,UVB累计照射剂量在0~10 J/cm2,按照最小红斑量(minimum erythema dose,MED)逐周递增方式进行造模,具有成模率高、重现性好以及与临床疾病高度吻合等优点。Objective To analyze the modeling elements and subjects of the animal model of skin photoaging,and to provide a reference for the preparation and improvement of the model and a basis for the scientific evaluation of the subject.Methods By searching and collecting relevant literature on the preparation of animal models of skin photoaging from 2010 to 2022 in the China National Knowledge Infrastructure,Wanfang Database,and PubMed database,the model animal species,gender,modeling method,modeling cycle,radiation source and its distance from the modeling site,cumulative radiation volume,detection indicators,and subjects(drugs or treatments)recorded in the literature were collated and summarized,and a database was established for statistical analysis.Results 257 articles that met the inclusion criteria were selected.Among them,the most common animal model was SKH-1 hairless mice,followed by SD rats and KM mice;the gender of animals was mainly female,medium-wave ultraviolet B(UVB)was often used as the radiation source,the distance between the radiation source and the modelling site was mostly 30 cm,and the modelling period was usually 40-60 days.The cumulative dose of long-wave ultraviolet A(UVA)was between 100-150 J/cm2,and the cumulative dose of UVB was between 5-10 J/cm2.The tests used after model establishment were skin histopathological examination,skin tissue homogenization,fibre staining,immunoblotting,etc.Subjects included Chinese herbal medicines,Chinese herbal extracts,Chinese patent medicines,Chinese herbal compound medicines,chemical drugs,biological agents and other treatments,while the animal model of skin photoaging was also used for clinical efficacy studies of external Chinese medicine,physiotherapy and positive control drugs.Conclusion In skin photoaging animal experiments,female SKH-1 hairless mice are often used,and UVB is used as the radiation source.The modeling period is usually 40-60 days,and the minimum erythema dose(MED)is incremented week by week.The cumulative UVB irradiation dose ranges from

关 键 词：皮肤光老化 动物模型 造模要素 受试物 SKH-1无毛小鼠 中波紫外线 

分 类 号：Q95-33[生物学—动物学] R-332[医药卫生]