纳米氧化亚铜和氧化铜的肺细胞毒性差异及影响因素  

Different Pulmonary Cytotoxicity of Nano-cuprous Oxide and Nano-copper Oxide and Influencing Factors

作　　者：汤蕾 李鑫 李昕畅 罗琳[1] 曹林英 Tang Lei;Li Xin;Li Xinchang;Luo Lin;Cao Linying(College of Resources and Environment,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]湖南农业大学资源环境学院,长沙410128

出　　处：《生态毒理学报》2023年第4期478-485,共8页Asian Journal of Ecotoxicology

基　　金：湖南省重点研发计划项目(2023NK2029);湖南省本科生/研究生科研创新项目(S202110537040,CX20220694);国家自然科学基金青年项目(21906050)。

摘　　要：纳米农药的广泛应用将不可避免导致其重要成分(比如纳米氧化铜和纳米氧化亚铜)的环境残留。人体可通过灰尘吸入、饮水及农产品摄入等方式暴露于纳米氧化铜和纳米氧化亚铜。因此,阐明它们的健康危害具有重要意义。本研究我们首次对比研究了纳米氧化铜和纳米氧化亚铜的A549肺细胞毒性差异,并从氧化应激和线粒体损伤角度探讨了细胞毒性作用机制。实验结果表明纳米氧化铜和纳米氧化亚铜均具有显著的A549细胞毒性,观察到的最低效应浓度分别为20 mg·L^(-1)和5 mg·L^(-1)。纳米氧化亚铜表现出更强的细胞毒性作用,100 mg·L^(-1)时细胞活性抑制率达到95%。活性氧实验结果表明纳米氧化铜和纳米氧化亚铜均可诱导活性氧生成,呈现时间与浓度依赖关系。纳米氧化亚铜的活性氧诱导作用明显强于纳米氧化铜,24 h最大诱导率分别为对照组的3.5倍和1.5倍。线粒体膜电位实验结果表明纳米氧化亚铜比纳米氧化铜具有更强的线粒体去极化作用,并呈剂量(1~100 mg·L^(-1))依赖关系,最大线粒体膜电位下降率分别为60%和20%。活性氧和线粒体膜电位的结果与细胞毒性的结果基本一致,提示了氧化应激和线粒体损伤可能是导致纳米氧化铜和纳米氧化亚铜细胞毒性的关键分子机制。本研究揭示了纳米氧化铜和纳米氧化亚铜的肺细胞毒性差异及潜在分子机制,可为纳米农药的健康风险评估及合理施用提供重要理论依据。The wide application of nano-pesticides leads to residues of its main components(such as nano-copper oxide and nano-cuprous oxide)in the environment inevitably.The human body can expose to nano-copper oxide and nano-cuprous oxide through inhaling dust,drinking water and ingesting agricultural products.Therefore,it is of great significance to elucidate their health hazards.In the present study,we firstly investigated the cytotoxicity variation of nano-copper oxide and nano-cuprous oxide on A549 cells as well as explored the cytotoxic mechanisms from the perspectives of oxidative stress and mitochondrial damage.Results showed that both nano-copper oxide and nano-cuprous oxide had significant cytotoxic effects on A549 cells,with the lowest observed effective concentrations of 20 mg·L^(-1) and 5 mg·L^(-1),respectively.Nano-cuprous oxide exhibited stronger cytotoxic effects than nano-copper oxide with inhibition rate of cell viability reaching 95%at 100 mg·L^(-1).The experimental results of reactive oxygen species showed that both nano-copper oxide and nano-cuprous oxide could induce the generation of reactive oxygen species in a time-dependent and concentration-dependent manner.The induction effect of nano-cuprous oxide on reactive oxygen species was significantly stronger than that of nano-copper oxide,with the maximum induction rate of 3.5-fold and 1.5-fold compared to the ctrl group at 24 h.The experimental results of mitochondrial membrane potential showed that nano-cuprous oxide had stronger activity on mitochondrial depolarization than nano-copper oxide in a dose-dependent manner(1~100 mg·L^(-1)),with the maximum reduction rates of mitochondrial membrane potential reaching 60%and 20%,respectively.The results of reactive oxygen species and mitochondrial membrane potential were consistent with the results of cytotoxicity,indicating that oxidative stress and mitochondrial damage might be the key molecular mechanisms of cytotoxicity of nano-copper oxide and nano-cuprous oxide.This study revealed the different p

关 键 词：纳米氧化铜 纳米氧化亚铜 肺细胞 细胞毒性 氧化应激 线粒体损伤 

分 类 号：X171.5[环境科学与工程—环境科学]