6-姜烯酚对鼻咽癌的作用及机制研究  

The effect and mechanism of 6-shogaol on nasopharyngeal carcinoma

作　　者：李泰运 廖钰 陈保平 周君怡 张敏 刘品月 LI Tai-yun;LIAO Yu;CHEN Bao-ping;ZHOU Jun-yi;ZHANG Min;LIU Pin-yue(School of Basic Medicine,Hunan University of Medicine,Huaihua,Hunan 418000)

机构地区：[1]湖南医药学院基础医学院,湖南怀化418000

出　　处：《赣南医学院学报》2023年第7期679-684,共6页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：国家大学生创新训练项目(201912214009)。

摘　　要：目的:探究6-姜烯酚对人鼻咽癌CNE2和HNE2细胞增殖、凋亡和迁移的影响并初步探究其机制。方法:6-姜烯酚处理组采用6-姜烯酚(25μmol·L^(-1))处理鼻咽癌CNE2和HNE2细胞,对照组加入同体积DMSO,空白组不做处理;48 h后,采用CCK-8法检测细胞增殖,流式细胞术检测细胞凋亡情况,细胞划痕实验检测细胞迁移能力,Western blot检测凋亡、增殖、上皮-间充质转化相关蛋白表达情况。结果:与空白组和对照组比较,6-姜烯酚处理组细胞增殖明显被抑制,凋亡细胞明显增加,PCNA蛋白表达水平明显降低,Caspase-3蛋白表达水平明显升高,差异有统计学意义(P<0.05);划痕实验划线48 h后,6-姜烯酚处理组的缝隙间距明显大于空白组和对照组,EMT相关蛋白表达水平显著改变,差异有统计学意义(P<0.05)。结论:6-姜烯酚可抑制CNE2和HNE2细胞增殖和迁移并促进其凋亡。Objective:To explore the effects of 6-shogaol on the proliferation,apoptosis and migration of human nasopharyngeal carcinoma CNE2 and HNE2 cells and its mechanism.Methods:CNE2 and HNE2 cells were treated with 6-gingerol(25μmol·L^(-1))in the experimental group,the same volume of DMSO was added to the control group;but the vacuity contrast group was not treated.After 48 hours,cell proliferation was detected by CCK-8 assay,cell apoptosis was detected by flow cytometry,cell migration ability was detected by cell scratches assay,and expression of proteins related to apoptosis,proliferation and epithelium-mesenchymal transformation were detected by Western Blot.Results:Compared with the control group and vacuity contrast group,cell proliferation of 6-shogaol experimental group was significantly inhibited,apoptosis was significantly increased,the expression level of PCNA protein was significantly decreased,and the expression level of Caspase-3 protein was significantly increased in the experimental group,with statistical significance(P<0.05).Forty-eight hours after the scratch experiment,the gap spacing of the experimental group was significantly greater than that of the control group and vacuity contrast group,and the expression level of EMT-related protein was significantly changed,with statistical significance(P<0.05).Conclusion:6-shogaol can inhibit the proliferation and migration of CNE2 and HNE2 cells and promote their apoptosis.

关 键 词：鼻咽癌 6-姜烯酚 HNE2细胞 CNE2细胞 

分 类 号：R739.6[医药卫生—肿瘤]