高聚合度菊粉通过调节肠-脂肪组织轴改善高脂饮食诱导的犬肥胖  

Inulin with High Degree of Polymerization Improves High-fat Diet Induced Obesity in Dogs by Regulating the Gut-adipose Tissue Axis

作　　者：陆江[1] 朱道仙 卢劲晔[1] 刘莉 郝福星 吴植 卢炜[1] 刘静[1] LU Jiang;ZHU Daoxian;LU Jinye;LIU Li;HAO Fuxing;WU Zhi;LU Wei;LIU Jing(Department of Pet Science and Technology,Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Department of Animal Medicine,Jiangsu Agri-animal Husbandry Vocational College,Taizhou 225300,China;Jiangsu Provincial Key Laboratory of Veterinary Bio-pharmaceutical High Tech Research,Taizhou 225300,China)

机构地区：[1]江苏农牧科技职业学院宠物科技学院,泰州225300 [2]江苏农牧科技职业学院动物医学院,泰州225300 [3]江苏省兽用生物制药高技术研究重点实验室,泰州225300

出　　处：《畜牧兽医学报》2023年第9期3941-3950,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省高校青蓝工程中青年带头人项目(苏教师函〔2021〕11号);江苏农牧科技职业学院校级课题(NSF2021ZR08)。

摘　　要：旨在基于肠-脂肪组织轴研究高聚合度菊粉改善高脂饮食诱导犬肥胖的作用机制。试验选取40只贵宾犬,随机等分为5组:正常饲粮组、高脂饲粮组和高聚合度菊粉低剂量组、中剂量组及高剂量组。正常饲粮组饲喂正常饲粮,高脂饲粮组饲喂高脂饲粮,高聚合度菊粉低剂量组、中剂量组及高剂量组分别饲喂含1.0%、3.0%和5.0%高聚合度菊粉的高脂饲粮,试验期为12周。试验结束后,测定血清糖脂和炎症因子含量,分别用实时荧光PCR检测和Western blot检测皮下脂肪组织和结肠黏膜中相关因子的mRNA相对表达和蛋白相对表达。结果表明:高聚合度菊粉可有效降低高脂饮食犬的体重、体脂率和血脂水平,改善糖耐量受损,提高结肠黏膜中闭锁蛋白(Occludin)和闭锁小带蛋白-1(ZO-1)的mRNA与蛋白表达量,降低血清脂多糖(LPS)、IL-6和TNF-ɑ水平以及脂肪组织中IL-6和TNF-ɑ蛋白表达。综上所述,高聚合度菊粉对高脂饮食诱导犬肥胖具有改善作用,其作用机制可能与调节“肠黏膜屏障功能-LPS易位-脂肪组织炎症”这一肠-脂肪组织轴有关。The aim of this study was to investigate the mechanism of inulin with high degree of polymerization in improving high-fat diet-induced obesity in dogs based on the gut-adipose tissue axis.Forty poodle were randomly divided into five groups:common diet group,high-fat diet group,and low-dose,medium-dose and high-dose inulin with high degree of polymerization groups.Dogs in common diet group were fed with normal diet,and in high-fat diet were fed with high-fat diet.Dogs in low-dose,medium-dose and high-dose inulin with high degree of polymerization groups were fed with high-fat diet containing 1.0%,3.0%and 5.0%inulin with high degree of polymerization,respectively.The test period was 12 weeks.After the experiment,the levels of serum glucose,fat and inflammatory factors were measured,and the relative expressions of mRNA and protein of related factors in subcutaneous adipose tissue and colon mucosa were detected using real-time fluorescent PCR and Western blot,respectively.The results showed as follows:Inulin with high degree of polymerization effectively reduced the body weight,body fat rate and blood lipid level,meanwhile improved the impaired glucose tolerance in dogs fed with high-fat diet.Moreover,inulin with high degree of polymerization increased the mRNA and protein expression of Occludin and ZO-1 in colonic mucosa,and reduced levels of serum lipopolysaccharide(LPS),IL-6 and TNF-ɑand the expression of IL-6 and TNF-ɑin adipose tissue.In conclusion,inulin with high degree of polymerization can improve the obesity induced by high-fat diet in dogs,and its mechanism may be related to the regulation of the gut-adipose tissue axis of"intestinal mucosal barrier function-LPS translocation-adipose tissue inflammation".

关 键 词：高聚合度菊粉 高脂饮食 犬 肥胖 肠-脂肪组织轴 

分 类 号：S856[农业科学—临床兽医学] S858.292[农业科学—兽医学]