长根菇液体深层发酵培养基的优化  

Optimization of liquid-submerged fermentation medium for Oudemansiella radicata

作　　者：梁锡坤 田腴澍 李心悦 胡君易 刘志锋 林晓霞 侯银臣 杨盛茹[2] 梁金明[1] LIANG Xikun;TIAN Yvshu;LI Xinyue;HU Junyi;LIU Zhifeng;LIN Xiaoxia;HOU Yinchen;YANG Shengru;LIANG Jinming(Zhongshan Agricultural Science and Technology Extension Center,Zhongshan Guangdong 528403;College of Food and Biology Engineering,Henan University of Animal Husbandry and Economy,Zhengzhou Henan 450046)

机构地区：[1]中山市农业科技推广中心,广东中山528403 [2]河南牧业经济学院食品与生物工程学院,河南郑州450046

出　　处：《现代牧业》2023年第3期38-43,55,共7页Modern Animal Husbandry

基　　金：中山市人才发展专项资金资助(ZX154791);河南省重点研发与推广专项(科技攻关)(212102110313)。

摘　　要：对长根菇液体深层发酵培养基成分进行优化。以长根菇菌丝生物量为指标,采用单因素试验对培养基中碳源、氮源及生长因子种类进行筛选,并考察不同含量的碳源、氮源及微量元素对菌丝生物量的影响。在单因素试验基础上,利用响应面试验优化深层发酵培养基中的碳源、氮源及微量元素的含量。结果表明,长根菇液体深层发酵的最佳培养基成分是脱脂麦胚粉26.52 g/L、酵母粉2.24 g/L、VB 60.10 g/L,在此条件下菌丝体的生物量为12.57 g/L。本研究确定了长根菇液体深层发酵培养基的最佳成分,为长根菇的人工种植提供了种子培养基础。The composition of liquid deep fermentation medium of Oudemansiella radicata was optimized.Taking the mycelium biomass of Oudemansiella radicata as the evaluation index,the optimal carbon source,nitrogen source and growth factor of the liquid medium were screened,and the effects of different contents of carbon and nitrogen sources and trace elements on mycelial biomass were investigated.The optimal fermentation medium conditions were optimized by response surface experiments.The result of research showed that the optimum medium composition for the liquid medium of Oudemansiella radicata were defatted wheat germ 26.52 g/L,yeast powder 2.24 g/L,vitamin B60.10 g/L,and the mycelium biomass of Oudemansiella radicata was up to 12.57 g/L under this optimized condition.In this paper,the optimum components of liquid deep fermentation medium were determined,which provided the seed culture basis for the artificial cultivation of Oudemansiella radicata.

关 键 词：长根菇 液体深层发酵 培养基 菌丝体 

分 类 号：Q815[生物学—生物工程]