免疫组织化学辅助纤维支气管镜活检在小细胞肺癌诊断中的应用效果  

Application Effect of Immunohistochemistry Assisted Fiber Bronchoscopy Biopsy for Diagnosis of Small Cell Lung Cancer

作　　者：徐晓梅 占承志 Xu Xiaomei;Zhan Chengzhi(Department of Pathology,Xianning Central Hospital,The First Affiliated Hospital of Hubei University of Science and Technology,Xianning 437000,Hubei Province,China)

机构地区：[1]咸宁市中心医院湖北科技学院附属第一医院病理科,湖北咸宁437000

出　　处：《中国社区医师》2023年第26期107-109,共3页Chinese Community Doctors

摘　　要：目的:探讨免疫组织化学(简称免疫组化)辅助纤维支气管镜(简称纤支镜)活检在小细胞肺癌诊断中的应用效果。方法:回顾性分析2019年8月—2022年8月于咸宁市中心医院经纤支镜活检确诊为小细胞肺癌患者72例的临床资料,采用免疫组化测定神经细胞黏附分子(CD56)、甲状腺转录因子-1(TTF-1)、广谱细胞角蛋白(CKpan)、嗜铬素A(CgA)、突触素(Syn)、增殖细胞核抗原Ki-67(Ki-67)的表达情况,分析免疫组化在小细胞肺癌病理诊断中的应用价值。结果:不同性别患者年龄、肿瘤组织分型、发病部位、病理分期比较,差异无统计学意义(P>0.05);男性吸烟比例高于女性,差异有统计学意义(P=0.001)。小细胞肺癌患者CD56、TTF-1、CKpan、CgA、Syn阳性率分别为77.78%(56/72)、72.22%(52/72)、69.44%(50/72)、70.83%(51/72)、77.78%(56/72),Ki-67指数≥60%者占69.44%(50/72)。广泛期患者Ki-67指数≥60%占比高于局限期患者,差异有统计学意义(P<0.05),其余不同病理特征患者之间各抗体比较差异均无统计学意义(P>0.05)。结论:免疫组化在纤支镜活检诊断小细胞肺癌病理中具有重要的应用价值,有助于鉴别其他肺原发性或转移性恶性肿瘤。Objective:To investigate the application effect of immunohistochemistry-assisted fiber bronchoscopy biopsy in the diagnosis of small cell lung cancer.Methods:The clinical data of 72 patients diagnosed with small cell lung cancer by fiber bronchoscopy biopsy in Xianning Central Hospital from August 2019 to August 2022 were retrospectively analyzed.The expressions of nerve cell adhesion molecule(CD56),thyroid transcription factor-1(TTF-1),broad-spectrum cytokeratin(CKpan),chromogranin A(CgA),synapsin(Syn),and proliferating nuclear antigen Ki-67(Ki-67)were determined by immunohistochemistry.The application value of immunohistochemistry in the pathological diagnosis of small cell lung cancer was analyzed.Results:There were no significant differences in age,tumor tissue typing,onset site and pathological stage between patients of different genders(P>0.05).The proportion of male smokers was higher than that of female smokers,and the difference was statistically significant(P=0.001).The positive rates of CD56,TTF-1,CKpan,CgA and Syn in patients with small cell lung cancer were 77.78%(56/72),72.22%(52/72),69.44%(50/72),70.83%(51/72),77.78%(56/72),and the proportion of Ki-67 index≥60%was 69.44%(50/72).The proportion of Ki-67 index≥60%in patients at extensive stage was higher than that in patients at local stage,and the difference was statistically significant(P<0.05).There was no significant difference in the antibodies among patients with different pathological features(P>0.05).Conclusion:Immunohistochemistry has important application value in the pathological diagnosis of small cell lung cancer by fiber bronchoscopy biopsy,and is helpful to identify other primary or metastatic malignant tumors of the lung.

关 键 词：免疫组织化学 纤维支气管镜 活检 小细胞肺癌 病理诊断 

分 类 号：R734.2[医药卫生—肿瘤]