铜绿假单胞菌诱导肺炎大鼠sST2/IL-33及Th17/Treg相关细胞因子表达  

Expressions of sST2/IL-33 and Th17/Treg-related cytokines in rats with Pseudomonas aeruginosa-induced pneumonia

作　　者：王晓静 杨洋[1] 滑红杰 李德保[3] 郭占元 WANG Xiao-jing;YANG Yang;HUA Hong-jie;LI De-bao;GUO Zhan-yuan(Jiyuan Vocational and Technical College,Jiyuan,Henan 459000,China;不详)

机构地区：[1]济源职业技术学院医学护理系,河南济源459000 [2]河南护理职业学院药学检验系,河南安阳455000 [3]焦作市人民医院检验科,河南焦作454150 [4]河南煤化焦煤集团中央医院检验科,河南焦作454000

出　　处：《中华医院感染学杂志》2023年第13期1921-1925,共5页Chinese Journal of Nosocomiology

基　　金：河南省医学教育指导性研究基金资助项目(Wjlx2020248)。

摘　　要：目的探究铜绿假单胞菌诱导肺炎大鼠可溶性生长刺激表达基因2(sST2)/白细胞介素(IL)-33及辅助性T细胞17(Th17)/调节性T细胞(Treg)相关细胞因子表达水平和意义。方法将60只SD大鼠随机分为空白对照组、肺炎12 h组、肺炎1 d组、肺炎3 d组、肺炎5 d组、肺炎7 d组各10只,除空白对照组外,其余大鼠均鼻滴铜绿假单胞菌菌液建立肺炎大鼠模型,检测大鼠肺功能指标,流式细胞术检测大鼠外周血Th17、Treg,收集大鼠支气管肺泡灌洗液(BALF),常规培养并计数菌落数、白细胞、中性粒细胞计数,酶联免疫法检测BALF中sST2、IL-33、IL-17、IL-23、肿瘤坏死因子α(TNF-α)、IL-1β水平并分析其相关性。结果与空白对照组比较,各肺炎组大鼠BALF菌落数、白细胞计数、中性粒细胞计数、BALF中sST2、IL-33、IL-17、IL-23、TNF-α、IL-1β、外周血Th17水平和Th17/Treg均为先升高后降低趋势(P<0.05),外周血Treg水平为先降低后升高趋势(P<0.05),于5 d达到最低(P<0.05),用力肺活量(FVC)、0.3 s用力呼吸容积(FEV0.3)降低(P<0.05);Pearson相关分析显示,各组大鼠BALF中sST2和IL-33水平分别与Th17/Treg、IL-17、IL-23、TNF-α、IL-1β水平呈正相关(P<0.05)。结论外周血sST2/IL-33可能通过调节Th17/Treg及相关细胞因子免疫平衡,参与铜绿假单胞菌诱发肺炎过程,在疾病急性发作期具有重要作用。OBJECTIVE To explore the expression levels of soluble growth stimulating gene 2(sST2)/interleukin-33(IL-33)and helper T cell 17(Th17)/regulatory T cell(Treg)-related cytokines in rats with pneumonia induced by Pseudomonas aeruginosa and observe the significance.METHODS Totally 60 SD rats were randomly divided into the blank control group,the 12-h pneumonia group,the 1-d pneumonia group,the 3-d pneumonia group,the 5-d pneumonia group and the 7-d pneumonia group,with 1o rats in each group.All of the rats were nasally dripped with P.aeruginosa to establish the rat models with pneumonia except for the rats in the blank control group.The lung function indexes of the rats were detected,the peripheral blood Th17 and Treg were detected by flow cytometry,the bronchoalveolar lavage fluid(BALF)specimens were collected from the rats so as to carry out the bacterial culture,the bacterial colony counts,white blood cell counts and neutrophil counts were recorded;the levels of sST2,IL-33,IL-17,IL-23,tumor necrosis factor-α(TNF-α)and IL-1βin BALF were detected by enzyme-linked immunosorbent assay,and the association between them was observed.RESULTS As compared with the blank control group,the bacterial colony counts,white blood cell counts,neutrophil counts in BALF and sST2,IL-33,IL-17,IL-23,TNF-αand IL-1βin BALF as well as peripheral blood Th17 and Th17/Treg were firstly elevated and then declined(P<0.05),while the peripheral blood Treg level firstly declined and then rose(P<0.05),reaching the lowest level on Day 5(P<0.05);the forced vital capacity(FVC)and forced expiratory volume in 0.3 second(FEV0.3)reduced(P<0.05).Pearson correlation analysis showed that the BALF sST2 and IL-33 were positively correlated with Th17/Treg,IL-17,IL-23,TNF-αand IL-1β,respectively(P<0.05).CONCLUSION The peripheral blood sST2/IL-33 may participate in the process of induction of pneumonia by P.aeruginosa by regulating the immune balance of Th17/Treg and related cytokines and play a significant role in acute attack of disease.

关 键 词：铜绿假单胞菌肺炎 大鼠 可溶性生长刺激表达基因2 白细胞介素-33 辅助性T细胞17/调节性T细胞细胞因子 

分 类 号：R563.1[医药卫生—呼吸系统]