机构地区:[1]南阳医学高等专科学校医学技术系,河南南阳473000 [2]南阳医学高等专科学校第一附属医院医学检验科,河南南阳473000 [3]南阳医学高等专科学校护理系,河南南阳473000 [4]南阳医学高等专科学校第一附属医院神经内科,河南南阳473000
出 处:《中华医院感染学杂志》2023年第13期1972-1975,共4页Chinese Journal of Nosocomiology
基 金:河南省卫生健康委员会基金资助项目(Wjlx2020270)。
摘 要:目的探讨γ-干扰素表面受体复合物(IFNGR1)基因多态性与成人慢性胃炎并发幽门螺杆菌(Hp)感染根除治疗后预后的关联。方法选择2018年1月-2020年3月南阳医学高等专科学校第一附属医院收治的慢性胃炎并发Hp感染患者239例为研究对象,所有患者均接受常规四联Hp根除方案治疗,疗程为10 d,疗程结束停药4~6周后进行根除疗效评估,据此将患者分为根除组136例和未根除组103例,收集患者临床资料,采用聚合酶链式扩增反应扩增IFNGR1基因rs1327475位点目的片段,采用酶联免疫吸附法检测血清中Hp特异性细胞毒素相关基因A(CagA)抗体,以临界值判断其毒力,并记录慢性胃炎并发Hp感染复发情况。结果未根除组与根除组IFNGR1基因rs1327475位点基因型、等位基因整体分布比较差异具有统计学意义(P<0.05),其中未根除组携带AA基因型、A等位基因频率高于根除组(P<0.05);未根除组携带IFNGR1基因AA基因型患者复发率高于携带GG/GA型患者(P<0.05),两组Hp负荷、Hp毒力亚型比较,无统计学差异。结论IFNGR1基因rs1327475位点多态性显著影响本地区慢性胃炎并发Hp感染人群根除治疗效果,且对疾病复发有一定影响。OBJECTIVE To explore the association of interferon-surface receptor complex(IFNGR1)gene polymorphisms with prognosis of chronic gastritis adults complicated with Helicobacter pylori(Hp)infection after eradication therapy.METHODS Totally 239 chronic gastritis adults complicated with Hp infection who were treated in the First Affiliated Hospital of Nanyang Medical College from Jan 2018 to Mar 2020 were recruited as the research subjects.All of the patients received the conventional quadruple Hp eradication program for treatment,with the course of treatment lo days.The effect of the eradication therapy was evaluated after the treatment course for 4-6 weeks.The patients were divided into the eradication group with 136 cases and the no eradication group with 103 cases according to the effect of eradication.The clinical data were collected form the patients,the target fragments at rs1327475 locus of IFNGR1 gene were amplified by polymerase chain reaction,the serum specific Hp cytotoxin-associated gene A(CagA)antibody was detected by means of enzyme-linked immunosorbent assay,the virulence was determined by the critical value,and the reoccurrence of Hp gastritis was recorded.RESULTS There were significant differences in the overall distributions of genotypes and alleles at rs1327475 locus of IFNGR1 gene between the eradication group and the no eradication group(P<0.05);the frequencies of AA genotype and A allele of the no eradication group were higher than those of the eradication group(P<o.05).In the no eradication group,the reoccurrence rate of the patients carrying with IFNGRl gen AA genotype was higher than that of the patients with carrying with GG/GA genotype(P<0.05).There were no significant differences in the Hp load and Hp virulence subtypes between the two groups.CONCLUSION The polymorphisms at rs1327475 locus of IFNGR1 gene remarkably affect the effect of eradication therapy among the population of chronic gastritis adults complicated with Hp infection in this area and have certain impact on the reoccurrence
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