Dectin-2基因多态性与乳腺癌根治术后继发侵袭性真菌感染的关联  

Association of Dectin-2 gene polymorphisms with postoperative secondary invasive fungal infection in patients undergoing radical mastectomy

作　　者：马玉 邹萌 王雪梅[3] 侯晓丹 任朗 MA Yu;ZOU Meng;WANG Xue-mei;HOU Xiao-dan;REN Lang(Shengjing Hospital Affiliated to China Medical University,Shenyang,Liaoning 110000,China)

机构地区：[1]中国医科大学附属盛京医院第一、二乳腺肿瘤外科病房,辽宁沈阳110000 [2]中国医科大学附属盛京医院第二普通外科胆道、整形美容外科病房,辽宁沈阳110000 [3]中国医科大学附属盛京医院第二泌尿外科,辽宁沈阳110000

出　　处：《中华医院感染学杂志》2023年第13期1996-1999,共4页Chinese Journal of Nosocomiology

基　　金：辽宁省科研基金资助项目(2020L02101)。

摘　　要：目的探究树突状细胞相关C型凝集素受体2(Dectin-2)基因多态性与乳腺癌根治术后继发侵袭性真菌感染(IFI)的关联。方法选取2019年1月-2021年6月于中国医科大学附属盛京医院接受根治术的乳腺癌患者124例,根据患者术后是否发生IFI分为IFI组39例和非IFI组85例。调查患者病历档案收集临床资料,分析患者术后发生IFI的危险因素。采用酶联免疫吸附法检测两组患者血清Dectin-2、白细胞介素-1β(IL-1β)和干扰素-γ(IFN-γ)水平,聚合酶链式反应-限制性片段长度多态性(PCR-EFLP)技术检测Dectin-2基因rs11045418位点单核苷酸多态性。结果IFI组合并糖尿病、住院时间≥10 d及进行辅助化疗占比高于非IFI组(P<0.05),但两组年龄、体质量指数(BMI)及高血压分布占比的比较,无统计学差异;Logistic回归模型分析显示,合并糖尿病、住院时间是乳腺癌患者根治术后继发IFI的危险因素(P<0.05);IFI组患者Dectin-2、IL-1β和IFN-γ水平高于非IFI组(P<0.05);rs11045418位点CC基因型是乳腺癌患者根治术后继发IFI的保护因素(P<0.05),但两组rs11045418位点基因型和等位基因分布频数的比较,无统计学差异。结论Dectin-2基因rs11045418位点CC基因型可能降低乳腺癌患者根治术后继发IFI风险,血清Dectin-2水平在IFI患者中也异常升高。OBJECTIVE To explore the association of dendritic cell-associated C-type lectin-2(Dectin-2)gene polymorphisms with postoperative secondary invasive fungal infection(IFI)in patients undergoing radical mastectomy.METHODS Totally 124 breast cancer patients who underwent radical mastectomy in Shengjing Hospital Affiliated to China Medical University from Jan 2019 to Jun 2021 were enrolled in the study and were divided into the IFI group with 39 cases and the non-IFI group with 85 cases according to the status of postoperative IFI.The medical records of the patients were investigated,the clinical data were collected,the risk factors for the postoperative IFI were analyzed.The levels of serum Dectin-2,interleukin-1β(IL-1β)and interferon-(IFN-)were detected for the two groups of patients by means of enzyme-linked immunosorbent assay,and the single nucleotide polymorphisms at rsi1045418 locus of Dectin-2 gene were detected by polymerase-chain-reaction-restriction fragment length polymorphism(PCR-RFLP)assay.RESULTS The proportions of patients with diabetes mellitus,length of hospital stay no less than io days and the frequency of adjuvant chemotherapy were higher in the IFI group than in the non-IFI group(P<0.05);there were no significant differences in the age,body mass index(BMI)and distribution frequency of hypertension between the two groups.Logistic regression model analysis showed that complication with diabetes mellitus and length of hospital stay were the risk factors for the postoperative secondary IFI in the breast cancer patients undergoing radical mastectomy(P<0.05).The levels of Dectin-2,IL-1βand IFN-of the IFI group were significantly higher than those of the non-IFI group(P<0.05);the CC genotype at rs11045418 locus was a protective factor for the postoperative secondary IFI(P<0.05);there were no significant differences in the frequencies of genotypes and alleles at rsl1045418 locus between the two groups.CONCLUSION The CC genotype at rsl1045418 locus of Dectin-2 gene may reduce the risk of postoperative

关 键 词：乳腺癌根治术 侵袭性真菌感染 树突状细胞相关C型凝集素受体2 危险因素 基因多态性 

分 类 号：R737.9[医药卫生—肿瘤]