肺癌患者KEAP1-NFE2L2基因相对拷贝数分析  

Relative Copy Number Analysis of KEAP1-NFE2L2 Gene in Lung Cancer Patients

作　　者：罗强[1] 兰碧洋[1] 黄斯阳[1] 张晓安[1] 武国煜 LUO Qiang;LAN Biyang;HUANG Siyang(Department of Cardio-Thoracic Gland,National Hospital of Guangxi Zhuang Autonomous Region,Nanning City,Guangxi 530001)

机构地区：[1]广西壮族自治区民族医院胸心腺体科,广西南宁市530001

出　　处：《医学理论与实践》2023年第19期3248-3251,3255,共5页The Journal of Medical Theory and Practice

基　　金：广西壮族自治区卫生健康委员会自筹经费科研课题(Z20210938)。

摘　　要：目的:探究KEAP1-NFE2L2基因相对拷贝数在肺癌发生发展机制中的作用,并分析KEAP1-NFE2L2基因相对拷贝数与患者的临床病理特征的关系。方法:采用实时荧光定量PCR方法检测肺癌患者和健康志愿者的血液样本中KEAP1-NFE2L2基因相对拷贝数和mRNA表达,分析它们在肺癌患者和健康志愿者之间的差异,同时利用Pearson相关性分析KEAP1-NFE2L2基因相对拷贝数和临床病理特征的关系。结果:肺癌患者NFE2L2以及MYC的相对拷贝数高于健康志愿者,差异有统计学意义(P<0.005)。肺癌患者中NFE2L2相对拷贝数与CYFRA21呈明显正相关(P=0.031),MYC拷贝数与KEAP1拷贝数呈正相关(P=0.008)。结论:MYC和NFE2L2的基因拷贝数可能和肺癌的发生和发展机制有关。Objective:To investigate the role of KEAP1-NFE2L2 gene relative copy number in the mechanism of lung cancer development and to analyze the relationship between KEAP1-NFE2L2 gene relative copy number and the clinicopathological characteristics of patients.Methods:The relative copy number and mRNA expression of KEAP1-NFE2L2 gene in blood samples from lung cancer patients and healthy volunteers were measured by real-time fluorescence quantitative PCR,and their differences between lung cancer patients and healthy volunteers were analyzed,while the relationship between the relative copy number of KEAP1-NFE2L2 gene and clinicopathological characteristics was analyzed using Pearson correlation.Results:The relative copy numbers of NFE2L2 as well as MYC were higher in lung cancer patients than in healthy volunteers,with statistically significant differences(P<0.005).The relative copy number of NFE2L2 and CYFRA21 were significantly positively correlated in lung cancer patients(P=0.031),and the copy number of MYC and KEAP1 were positively correlated(P=0.008).Conclusion:Gene copy number of MYC and NFE2L2 may be related to the mechanism of lung cancer development and progression.

关 键 词：MYC NFE2L2 肺癌 基因相对拷贝数 

分 类 号：R734.2[医药卫生—肿瘤]