血管内皮生长因子D、miRNA-34在甲状腺乳头状癌中的表达及与患者预后的关系  

Expression of vascular endothelial growth factor D and miRNA-34 in papillary thyroid carcinoma and their relationship with prognosis

作　　者：张青松[1] 杨迎旭 谌童童 ZHANG Qingsong;YANG Yingxu;CHEN Tongtong(Department of Thyroid Surgery,Zhengzhou Central Hospital,Zhengzhou 450000,He’nan,China)

机构地区：[1]郑州市中心医院甲状腺外科,郑州450000

出　　处：《癌症进展》2023年第14期1539-1542,共4页Oncology Progress

基　　金：2018年度河南省医学科技攻关计划项目(2018020789)。

摘　　要：目的 探讨血管内皮生长因子D(VEGFD)、miRNA-34在甲状腺乳头状癌中的表达及与患者预后的关系。方法 取352例甲状腺乳头状癌患者的甲状腺乳头状癌组织及349例甲状腺良性病变患者的正常甲状腺组织,采用免疫组化法检测VEGFD的表达情况,逆转录聚合酶链反应(RT-PCR)法检测miRNA-34的表达情况。甲状腺乳头状癌患者预后不良的影响因素采用多因素Logistic回归分析。结果 甲状腺乳头状癌组织中VEGFD、miRNA-34阳性表达率均明显高于正常甲状腺组织,差异均有统计学意义(P﹤0.01)。随访3年,352例甲状腺乳头状癌患者中预后良好319例,预后不良33例,分别作为预后良好组和预后不良组,预后良好组和预后不良组甲状腺乳头状癌患者淋巴结转移情况、VEGFD表达情况、miRNA-34表达情况比较,差异均有统计学意义(P﹤0.05)。多因素Logistic回归分析结果显示,淋巴结转移、VEGFD阳性表达、miRNA-34阳性表达均是甲状腺乳头状癌患者预后不良的独立危险因素(P﹤0.05)。结论 甲状腺乳头状癌组织中VEGFD、miRNA-34均呈高表达,且与患者的预后密切相关,淋巴结转移、VEGFD阳性表达、miRNA-34阳性表达均是甲状腺乳头状癌患者预后不良的独立危险因素。Objective To explore the expression of vascular endothelial growth factor D(VEGFD)and miRNA-34 in papillary thyroid carcinoma and their relationship with prognosis.Method The papillary thyroid carcinoma tissues from 352 patients with papillary thyroid carcinoma and normal thyroid tissues from 349 patients with benign thyroid lesions were selected.The expression of VEGFD was detected by immunohistochemistry,and the expression of miRNA-34 was detected by reverse transcription-polymerase chain reaction(RT-PCR).The influencing factors of poor prognosis in pa-tients with papillary thyroid carcinoma were analyzed using multivariate Logistic regression.Result The positive expres-sion rates of VEGFD and miRNA-34 in papillary thyroid carcinoma tissues were significantly higher than those in normal thyroid tissues,and the differences were statistically significant(P<0.01).Following up for 3 years,among 352 patients with papillary thyroid carcinoma,319 had good prognosis and 33 had poor prognosis,they were divided into good prog-nosis group and poor prognosis group,respectively.There were statistically significant differences in lymph node metasta-sis,VEGFD expression,and miRNA-34 expression between the good prognosis group and the poor prognosis group(P<0.05).Multivariate Logistic regression analysis showed that lymph node metastasis,positive expression of VEGFD,and positive expression of miRNA-34 were independent risk factors for poor prognosis in patients with papillary thyroid carci-noma(P<0.05).Conclusion VEGFD and miRNA-34 are highly expressed in papillary thyroid carcinoma tissues and closely related to patient prognosis.Lymph node metastasis,positive expression of VEGFD,and positive expression of miRNA-34 are independent risk factors for poor prognosis in papillary thyroid carcinoma patients.

关 键 词：甲状腺乳头状癌 血管内皮生长因子D miRNA-34 预后 

分 类 号：R736.1[医药卫生—肿瘤]