赛里木酸奶中乳酸菌的分离鉴定及特性分析  

Screening, identification and characteristics ofLactic acid bacteria from the sayram ketteki

作　　者：何齐 冯倩 李雪 易鸳鸯 顾美英[2] 朱静[2] 孙建[2] 张志东 HE Qi;FENG Qian;LI Xue;YI Yuanyang;GU Meiying;ZHU Jin;SUN Jian;ZHANG Zhidong(College of Food Sciences and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,China;Xinjiang Laboratory of Special Environmental Microbiology/Institute of Applied Microbiology,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)

机构地区：[1]新疆农业大学食品科学与药学学院,乌鲁木齐830052 [2]新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐830091

出　　处：《新疆农业科学》2023年第9期2323-2330,共8页Xinjiang Agricultural Sciences

基　　金：新疆区域协同创新专项(上海合作组织科技伙伴计划)(2020E01007);国家重点研发计划项目(2018YFC1603400;2018YFC1603404)。

摘　　要：【目的】挖掘和利用赛里木酸奶中的乳酸菌资源。【方法】采集12份阿克苏地区赛里木镇周边酸奶样品,采用平板稀释法进行菌株分离,通过16S rRNA基因序列测序和菌株形态学观察和明确菌株分类学地位,检测菌株溶血性和有害代谢物质等。【结果】在12种赛里木酸奶样品中筛选得到4种乳酸菌,分属于Eremococcus、Leuconostoc、Lentilactobacillus、Streptococcus 4个属下的4个种,试验菌株可耐受0.5%胆盐,0.5%NaCl,且具有较好耐高温、牛奶胨化和抑制细菌等特性,并展现出良好的自聚合能力;所有菌株均无溶血现象;除H5-4外利用氨基酸均不产生物胺,并不产生硝基还原酶。【结论】从赛里木酸奶中分离获得的乳酸菌具有良好的发酵特性和食品安全性。【Objective】To explore and utilize the LAB resources in Sayram Ketteki.【Methods】12 yoghurt samples were collected around Sayram town in Aksu Prefecture,and the strains were isolated by the plate dilution method.The 16S rRNA gene sequences and morphological observation were carried out to clarify the taxonomic status of the strains,and edible safety characteristics such as hemolysis and harmful metabolites were detected.【Results】A total of 36 LAB strains were isolated and identified,which belonged to 4 species of Eremococcus,Leuconostoc,Lentilactobacillus and Streptococcus;the tested strains could tolerate 0.5%bile salt,5%NaCl,and showed good high temperature resistance,milk peptonization,different enzyme activities,antibacterial properties,and auto aggregation ability.None of strains were positive for hemolysis.Nonitroreductase and biogenic amines were produced by strains except H5-4.【Conclusion】The LAB obtained from camel milk in this study have good fermentation characteristics and food safety,which provides a research basis for the subsequent development of lactic acid bacteria resources.

关 键 词：赛里木酸奶 乳酸菌 分离筛选 

分 类 号：S188[农业科学—农业基础科学] S879.1