癌相关成纤维细胞外泌体miR-34c-5p对胃癌细胞干细胞样表型的影响  

Effect of Cancer-associated Fibroblast Exosomal miR-34c-5p on GastricCancer Stem Cell-like Phenotype

作　　者：徐秀连[1] 谢平[1] 印隆宽 袁华燕 刘建军[1] 王攀[1] XU Xiulian;XIE Ping;YIN Longkuan;YUAN Huayan;LIU Jianjun;WANG Pan(Department of Gastrointestinal Surgery,Affiliated Hospital of North Sichuan Medical College,Nanchong,637000,China)

机构地区：[1]川北医学院附属医院胃肠外一科,四川省南充市637000

出　　处：《医学分子生物学杂志》2023年第5期432-438,共7页Journal of Medical Molecular Biology

基　　金：南充市科技项目应用基础课题研究(No.19SXHZ0323)。

摘　　要：目的探讨癌相关成纤维细胞(cancer-associated fibroblasts,CAFs)来源的外泌体miR-34c-5p是否有助于促进胃癌(gastric carcinoma,GC)细胞的干细胞样特性。方法收集并鉴定初代培养的CAFs和配对的正常成纤维细胞(normol fibroblasts,NFs)的外泌体,检测CAFs和NFs之间外泌体中miR-34c-5p的差异表达。CCK8实验检测GC细胞活力;克隆形成实验检测GC细胞增殖能力;Transwell实验检测GC细胞侵袭能力;细胞成球形成实验评估GC细胞成球能力;蛋白质印迹法检测细胞干细胞特性相关蛋白的表达。结果miR-34c-5p在CAFs衍生的外泌体中的表达显著降低。与CAFs-exo组比较,CAFs-exo中miR-34c-5p抑制GC细胞的增殖和侵袭能力(P<0.05);抑制GC细胞成球,并降低SOX2、OCT4和NANOG蛋白的表达水平,有显著性差异(P<0.05)。结论CAFs来源的外泌体miR-34c-5p抑制GC细胞的干细胞样表型。Objective To investigate the effect of exosomal miR-34c-5p derived from cancerassociated fibroblasts(CAFs)on the stem-like properties of gastric cancer(GC)cells.Methods Exosomes from primary cultured CAFs and paired normal fibroblasts(NFs)were collected and identified,and the differential expression of exosomal miR-34c-5p between CAFs and NFs was detected.The viability of GC cells was detected by CCK8 assay.The proliferation of GC cells was detected by colony formation assay.The invasive ability of GC cells was detected by Transwell assay.The sphere formation capability of GC cells was evaluated by sphere formation assay.The expression levels of stemness-related proteins were detected by Western blotting(WB)assay.Results The expression level of miR-34c-5p in CAFs derived exosomes was significantly decreased.miR-34c-5p from the CAFs-exo inhibited the proliferation,invasion and sphere formation of GC cells,and reduced the expression levels of SOX2,OCT4 and NANOG proteins,when compared with those in the CAFs-exo group(P<0.05).Conclusion The exosomal miR-34c-5p from CAFs inhibited the stem-like properties of GC cells.

关 键 词：癌相关成纤维细胞 外泌体 胃癌 miR-34c-5p 干细胞特性 

分 类 号：R735.2[医药卫生—肿瘤]