豫南地区犬细小病毒流行株遗传变异分析  

Analysis of genetic variation of canine Parvovirus epidemic strains in southern Henan Province

作　　者：鲁绍芳 武娴 郭晓秋[1,3] 董丽娜 余文静 张玮 扶晓 冉梦媛 刘涛 赵云焕[1,3] 曲哲会 LU Shaofang;WU Xian;GUO Xiaoqiu;DONG Li’na;YU Wenjing;ZHANG Wei;FU Xiao;RAN Mengyuan;LIU Tao;ZHAO Yunhuan;QU Zhehui(College of Animal Science and Technology,Xinyang Agriculture and Forestry University,Xinyang 464000,China;Xinyang City Key Laboratory of Comprehensive Technology Research for the Prevention and Control of Major Livestock and Poultry Diseases,Xinyang 464000,China;Henan Provincial Dabieshan District Engineering Research Center for Health Production of Ecological Livestock and Poultry,Xinyang 464000,China)

机构地区：[1]信阳农林学院动物科技学院,河南信阳464000 [2]信阳市畜禽重大疫病防控综合技术研究重点实验室,河南信阳464000 [3]河南省大别山区生态畜禽健康生产工程研究中心,河南信阳464000

出　　处：《黑龙江畜牧兽医》2023年第17期91-97,共7页Heilongjiang Animal Science And veterinary Medicine

基　　金：信阳农林学院高水平科研孵化器建设项目(FCL202004);信阳农林学院青年科研基金项目(QN2021012)。

摘　　要：为了了解豫南地区犬细小病毒(canine Parvovirus,CPV)流行株的遗传变异特征,试验利用PCR方法从临床采集的CPV阳性犬的粪便中扩增CPV的VP2和NS1基因,并测定核苷酸序列,利用软件Meglign 7.0推导VP2和NS1基因的氨基酸序列并构建进化树,进行同源性和氨基酸变异分析。结果表明:从8份粪便样品中分离得到8株CPV流行株,分别命名为CH-XY-1~CH-XY-8。基于VP2基因推导的氨基酸序列,豫南地区流行株CH-XY-7和CH-XY-8为New CPV-2a亚型,CH-XY-5为New CPV-2b亚型,CH-XY-1、CH-XY-2、CH-XY-3、CH-XY-4和CH-XY-6为CPV-2c亚型,CH-XY-3和CH-XY-6分别存在1个(G299R)和2个(V84E和V153A)新的氨基酸变异。基于NS1基因推导的氨基酸序列,流行株CH-XY-5、CH-XY-6、CH-XY-7和CH-XY-8分布在GroupⅠ群,其中CH-XY-6和CH-XY-8分别存在1个新的氨基酸变异位点,分别为N356K和E583K;CH-XY-1、CH-XY-2、CH-XY-3和CH-XY-4分布在GroupⅡ群,CH-XY-3存在5个新的氨基酸变异,分别为N23S、F30L、N38D、R61G和Q307H。说明豫南地区流行的CPV出现CPV-2型多种亚型并存的情况,且已经发生变异。In order to understand the genetic variation characteristics of canine Parvovirus(CPV)in southern Henan Province,PCR method was used to amplify the VP2 and NS1 genes of CPV in feces from clinically CPV-infected dogs,and the nucleotide sequences were determined.The phylogenetic tree construction,homology and amino acid variation analysis of the deduced VP2 and NS1 amino acid sequences were performed using the software Meglign 7.0.Based on the deduced amino acid sequences analysis of VP2 gene,it showed that the epidemic strains CH-XY-7 and CH-XY-8 were New CPV-2a genotype.The epidemic strain CH-XY-5 was New CPV-2b genotype,and the epidemic strain CH-XY-1,CH-XY-2,CH-XY-3,CH-XY-4 and CH-XY-6 were CPV-2c genotype.There were 1(G299R)and 2(V84E and V153A)new variations in VP2 amino acids of epidemic strain CH-XY-3 and CH-XY-6,respectively.Based on the deduced amino acid sequences analysis of the NS1 gene,it showed that the epidemic strains CH-XY-5,CH-XY-6,CH-XY-7 and CH-XY-8 were divided.Into GroupⅠ,with CH-XY-6 and CH-XY-8 each exhibiting a new amino acid variation(N356K in CH-XY-6,E583K in CH-XY-8),CH-XY-1,CHXY-2,CH-XY-3 and CH-XY-4 were divided into GroupⅡ,and there are 5 new amino acid variants in epidemic strain CH-XY-3(N23S,F30L,N38D,R61G and Q307H).It showed that the CPV prevalent in the southern Henan Province coexisted with multiple subtypes of CPV-2 and had mutated.

关 键 词：犬细小病毒 VP2基因 NS1基因 遗传变异分析 豫南地区 

分 类 号：S852.659.2[农业科学—基础兽医学]