姜黄素对罗曼蛋鸡产蛋性能、卵泡发育和抗氧化功能的影响  

Effects of curcumin on egg laying performance,follicle development and anti-oxidation function of Roman laying hens

作　　者：夏卿 高文 陈玉霞 谢婷婷 张丽[1] 效梅[1] 安立龙[1] XIA Qing;GAO Wen;CHEN Yuxia;XIE Tingting;ZHANG Li;XIAO Mei;AN Lilong(College of Coastal Agricultural Sciences,Guangdong Province Characteristic Poultry Ecological Health Breeding Science and Technology Innovation Center,Guangdong Ocean University,Zhanjiang 524000,China)

机构地区：[1]广东海洋大学滨海农业学院广东省特色家禽生态健康养殖科技创新中心,广东湛江524000

出　　处：《黑龙江畜牧兽医》2023年第17期98-103,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：广东省自然科学基金粤桂联合基金重点项目(2020B1515420008)。

摘　　要：为了解姜黄素对罗曼蛋鸡产蛋性能、卵泡发育和抗氧化功能的影响,试验将健康的26周龄生产性能相近的144只雌性罗曼蛋鸡随机分为4组,分别为对照组(NC组)、NT1组、NT2组和NT3组,每组6个重复,每个重复6只。NC组饲喂不含姜黄素的基础日粮,NT1组、NT2组和NT3组分别饲喂添加50,150,250 mg/kg姜黄素的基础日粮,预试期7 d,正试期63 d。正试期每天统计各组产蛋性能指标。试验结束后测定各级卵泡数量,通过ELISA法测定血清生殖激素水平及抗氧化指标[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)],运用实时荧光定量PCR法测定卵巢FSH受体(FSHR)、LH受体(LHR)、E2受体(ER)和P4受体(PR)的mRNA相对表达量。结果表明:NT2组的产蛋率和小白卵泡(SWF)数量显著高于NC组和NT1组(P<0.05);NT2组和NT3组的FSH、LH、E2及P4水平显著高于NC组和NT1组(P<0.05),其中NT2组和NT3组之间FSH、LH及P4水平差异不显著(P>0.05),NT2组FSH和LH水平的提升效果更佳,NT3组E2和P4水平的提升效果更佳;与NC组相比,NT2组卵巢FSHR、LHR、ER和PR的mRNA相对表达量显著提高(P<0.05);NT2组和NT3组SOD、CAT及GSH-Px活性显著高于NC组和NT1组(P<0.05),MDA浓度显著低于NC组和NT1组(P<0.05)。说明在日粮中添加姜黄素可以提高罗曼蛋鸡抗氧化功能、卵巢生殖激素受体mRNA相对表达量、血清生殖激素水平和SWF数量,进而提高产蛋率,在本试验条件下,考虑经济因素,以150 mg/kg为姜黄素的最适添加量。In order to explore the effects of curcumin on egg laying performance,follicular development and antioxidant function in Roman laying hens,a total of 14426-week-old healthy female Roman laying hens with similar performance were randomly divided into four groups:control group(NC),NT1 group,NT2 group and NT3 group.Each group had 6 replicates and each replicate had 6 hens.The animals in the control group(NC)were fed a basal diet without curcumin;the animals in the NT1 group,NT2 group and NT3 group were fed the basal diet supplemented with curcumin containing 50 mg/kg,150 mg/kg and 250 mg/kg.The pre-trial period lasted for 7 days and the experimental period lasted for 63 days.The egg laying performance indicators of each group were counted every day during the current test.After the end of the test,the number of follicles at all levels was measured;the serum reproductive hormone level and the antioxidant index(superoxide dismutase[SOD],catalase[CAT],GSH peroxidase[GSH-Px]and malondialdehyde[MDA])were determined by ELISA,and the relative mRNA expression levels of ovarian FSH receptor(FSHR),LH receptor(LHR),E2 receptor(ER)and P4 receptor(PR)were measured by real-time fluorescence quantitative PCR.The results showed that the laying rate and the number of small white follicles(SWF)in the NT2 group were significantly higher than those in the NC group and NT1 group(P<0.05).The levels of FSH,LH,E2 and P4 in the NT2 and NT3 groups were significantly higher than those of the NC group and NT1 group(P<0.05),and there was no significant difference in the levels of FSH,LH and P4 between the NT2 and NT3 groups(P>0.05).The increase of FSH and LH levels in the NT2 group was better,and the increase of E2 and P4 levels in the NT3 group was better.The relative mRNA levels of ovarian FSHR,LHR,ER and PR was significantly increased in the NT2 group compared with the NC group(P<0.05).The activities of SOD,CAT,and GSH-Px in the NT2 and NT3 groups were significantly higher than those in NC and NT1 groups(P<0.05),and the MDA concentration was

关 键 词：姜黄素 罗曼蛋鸡 卵泡发育 生殖激素 抗氧化功能 

分 类 号：S816.7[农业科学—饲料科学]