HK2在初治急性髓系白血病患者骨髓中的表达及对预后的影响  

Expression and Prognostic Impact of HK2 in Bone Marrow of Patients with Newly Diagnosed Acute Myeloid Leukemia

作　　者：马平[1] 史利欢 田亮[1] 王亚峰[1] 周建文[1] 毛彦娜[1] 刘炜[1] 侯玉玮 孙慧[3] MA Ping;SHI Li-Huan;TIAN Liang;WANG Ya-Feng;ZHOU Jian-Wen;MAO Yan-Na;LIU Wei;HOU Yu-Wei;SUN Hui(Department of Hematology and Oncology,Children′s Hospital Affiliated to Zhengzhou University,Henan Children′s Hospital,Zhengzhou Children′s Hospital;Children′s Hospital Affiliated to Zhengzhou University,Henan Key Laboratory of Children′s Genetics and Metabolic Diseases;Department of Hematology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,Henan Province,China)

机构地区：[1]郑州大学附属儿童医院/河南省儿童医院/郑州儿童医院血液肿瘤科 [2]郑州大学附属儿童医院/河南省儿童遗传代谢性疾病重点实验室 [3]郑州大学第一附属医院血液科,河南郑州450000

出　　处：《中国实验血液学杂志》2023年第5期1309-1314,共6页Journal of Experimental Hematology

基　　金：河南省科技攻关(212102310036)。

摘　　要：目的:检测HK2基因在初治急性髓系白血病(AML)骨髓中的表达水平并探讨其对患者临床特征及预后的影响。方法:采用RT-q PCR法检测90例初治AML患者骨髓中HK2基因的表达情况,并与18例异基因造血干细胞移植供者进行对比,进一步分析HK2基因的表达水平与临床特征及预后的关系。结果:相较于正常对照组,初治AML组的HK2基因表达量显著增高(P<0.01);相较于2个疗程诱导化疗达到总反应(OR,完全缓解+完全缓解伴血细胞不完全恢复)的患者,未达到OR的患者HK2基因的表达量显著增高(P<0.05);2个疗程诱导治疗是否达到OR的患者HK2基因相对表达量比较存在显著差异(P<0.001);HK2高表达患者中位生存时间显著短于HK2低表达患者(P<0.05);Cox多因素分析结果显示,预后分层、HK2基因表达量、2个疗程诱导治疗是否达到OR均是AML患者预后的独立影响因素(P<0.05)。结论:初治AML患者骨髓中HK2基因的表达水平相较于异基因造血干细胞移植供者显著升高,高表达HK2基因患者预后较差,HK2基因表达量是AML患者预后的独立影响因素。Objective:To detect the expression level of HK2 gene in the bone marrow of newly diagnosed patients with acute myeloid leukemia(AML)and investigate its influence on the clinical characteristics and prognosis.Methods:The expression level of HK2 gene in the bone marrow of 90 newly diagnosed patients with AML that accompanying clinical characteristics and survival status were detected by RT-qPCR,and compared with 18 allogeneic hematopoietic stem cell transplantation(allo-HSCT)donors.The Chi-square test,Kaplan-Meier survival analysis,and Cox proportional hazards regression model were used to analyze the correlation of HK2 expression level with clinical characteristics and prognosis.Results:Compared with allo-HSCT donors,the HK2 expression was significantly increased in newly diagnosed AML patients(P<0.01).Compared with patients with total response(OR,complete response+complete response with incomplete hematologic recovery)after 2 courses of induction chemotherapy,the expression of HK2 in patients without OR was significantly increased(P<0.05).There was a significant difference in the relative expression of HK2 between patients with and without OR after 2 courses of induction therapy(P<0.001).The median survival time of patients with high expression of HK2 was significantly shorter than that of patients with low expression of HK2(P<0.05).The multivariate Cox proportional hazards regression analysis showed that prognostic stratification,the expression level of HK2,and whether two courses of induction therapy achieved OR were independent factors affecting the prognosis of AML patients(P<0.05).Conclusions:Compared with allo-HSCT donors,the expression level of HK2 gene is increased in the bone marrow of newly diagnosed AML patients.The prognosis of patients with high expression of HK2 is poor.The expression level of HK2 is an independent factor affecting the prognosis of AML patients.

关 键 词：HK2基因 急性髓系白血病 预后 

分 类 号：R733.71[医药卫生—肿瘤]