多发性骨髓瘤浆细胞CD117/CD200表达模式与分子遗传学预后参数的相关性研究  

Correlation between Expression Patterns of CD117/CD200 in Plasma Cells and Molecular Genetic Prognostic Parameters in Multiple Myeloma

作　　者：汪平 王学东 刘露[2] 陈肖 刘芳[2] 赵四书 杨慧[2] 仇海荣[2] 郭睿[2] 李建勇[2] 吴雨洁[2] WANG Ping;WANG Xue-Dong;LIU Lu;CHEN Xiao;LIU Fang;ZHAO Si-Shu;YANG Hui;QIU Hai-Rong;GUO Rui;LI Jian-Yong;WU Yu-Jie(Anhui No.2 Provincial People’s Hospital,Hefei 230011,Anhui Province China;Department of Hematology,The First Affiliated Hospital of Nanjing Medical University,Jiangsu Provincial People’s Hospital,Nanjing 210029,Jiangsu Province,China)

机构地区：[1]安徽省第二人民医院医学检验中心,安徽合肥230011 [2]南京医科大学第一附属医院,江苏省人民医院血液科,江苏南京210029

出　　处：《中国实验血液学杂志》2023年第5期1415-1420,共6页Journal of Experimental Hematology

摘　　要：目的:探讨多发性骨髓瘤(MM)患者浆细胞CD117和CD200的表达与分子遗传学异常的相关性。方法:选取初诊MM患者100例,采集患者新鲜骨髓液,利用流式细胞术(FCM)和荧光原位杂交(FISH)技术检测浆细胞免疫表型和染色体结构变异。结果:CD117和CD200在所有MM患者异常浆细胞中的阳性表达率均为44.0%(44/100),75例行FISH检测的患者中有53例至少检出1种分子遗传学异常,总检出率为70.7%(53/75),1q21(CKS1B)基因复制、1p32(CDKN2C)基因缺失、p53基因缺失、IgH重排检出率分别为48.6%(36/74)、10.6%(7/66)、11.1%(8/72)、32.9%(24/73)。CD117^(+)患者IgH重排发生率明显低于CD117^(-)患者(P<0.05),CD200^(-)患者1p32(CDKN2C)基因缺失比例明显低于CD200^(+)患者(P<0.05)。根据CD117及CD200的表达,将患者分成4组(CD117^(+)CD200^(+)、CD117^(+)CD200^(-)、CD117^(-)CD200^(+)、CD117^(-)CD200^(-)),进一步分析发现CD117^(+)CD200^(-)组IgH重排发生率明显低于CD117^(-)CD200^(+)组(P<0.05),CD117^(+)CD200^(-)组1p32(CDKN2C)基因缺失率明显低于CD117^(+)CD200^(+)组和CD117^(-)CD200^(+)组(P<0.05)。结论:CD117联合CD200表达模式的不同对MM患者的预后判断有重要价值,肿瘤细胞具有CD117^(-)CD200^(+)表达模式的MM患者预后更差。Objective:To investigate the correlation between the expression of CD117 and CD200 in plasma cells and molecular genetic abnormalities in patients with multiple myeloma(MM).Methods:100 newly diagnosed MM patients were selected,and fresh bone marrow fluid was collected from the patients.The immunophenotypes and chromosomal structural variations of plasma cells were detected by flow cytometry(FCM)and fluorescence in situ hybridization(FISH).Results:The positive expression frequencies of CD117 and CD200 in abnormal plasma cells of all MM patients were 44.0%and 44.0%,respectively.At least one molecular genetic abnormality was detected in 53 of the 75 patients who underwent FISH testing,and the overall detection rate was 70.7%(53/75).The detection rates of 1 q21(CKS1B)duplication,1 p32(CDKN2C)deletion,p53 deletion and IgH rearrangement were 48.6%(36/74),10.6%(7/66),11.1%(8/72)and 32.9%(24/73),respectively.The incidence of IgH rearrangement in CD117^(+)patients was significantly lower than that in CD117-patients(P<0.05),and the proportion of 1 p32(CDKN2C)deletion in CD200^(-)patients was significantly lower than that in CD200^(+)patients(P<0.05).According to the expressions of CD117 and CD200,the patients were divided into 4 groups:CD117^(+)CD200^(+),CD117^(+)CD200^(-),CD117-CD200^(+)and CD117-CD200^(-).Further analysis showed that the incidence of IgH rearrangement in the CD117^(+)CD200^(-)group was significantly lower than that in the CD117-CD200^(+)group(P<0.05),and the deletion rate of 1 p32(CDKN2C)gene in CD117^(+)CD200^(-)group was significantly lower than that in CD117^(+)CD200^(+)group and CD117-CD200^(+)group(P<0.05).Conclusion:The difference in the expression patterns of CD117 combined with CD200 shows important value in judging the prognosis of MM patients,and the MM patients with CD117-CD200^(+)expression patterns in abnormal plasma cells have a worse prognosis.

关 键 词：多发性骨髓瘤 CD117 CD200 荧光原位杂交 

分 类 号：R733.3[医药卫生—肿瘤]