MiR⁃320通过调节JAK2/STAT3和NF⁃κB信号通路对急性胰腺炎大鼠肠道损伤的影响  

Effect of MiR⁃320 on Intestinal Injury in Rats With Acute Pancreatitis by Regulating JAK2/STAT3 and NF⁃κB Signaling Pathways

作　　者：冯文静[1] 穆静辉[1] 李彦泽[2] 程敏静[3] 李洁[4] 梁玉龙[3] FENG Wenjing;MU Jinghui;LI Yanze;CHENG Minjing;LI Jie;LIANG Yulong(Department of General Surgery,the Third Hospital of Hebei Medical University,Shijiazhuang,050051;Department of Vascular Surgery,the Third Hospital of Hebei Medical University,Shijiazhuang,050051;Department of Gastrointestinal Surgery,the Third Hospital of Hebei Medical University,Shijiazhuang,050051;Department of Thoracic Surgery,the Third Hospital of Hebei Medical University,Shijiazhuang,050051)

机构地区：[1]河北医科大学第三医院普通外科,050051 [2]河北医科大学第三医院血管外科,050051 [3]河北医科大学第三医院胃肠外科,050051 [4]河北医科大学第三医院胸外科,050051

出　　处：《胃肠病学》2022年第10期589-595,共7页Chinese Journal of Gastroenterology

基　　金：2022年河北省卫健委科研基金项目(20221171)。

摘　　要：背景:微RNA-320(mi R-320)表达在急性胰腺炎中下调,对急性胰腺炎的影响机制尚不完全清楚。目的:探讨mi R-320对急性胰腺炎大鼠肠道损伤的影响及其机制。方法:将大鼠随机分为假手术组、模型组、mi R-320激动剂组(agomir mi R-320组)、mi R-320激动剂对照组(agomir NC组)、JAK2抑制剂组(AG490组)、NF-κB通路抑制剂组(PDTC组),采用逆行胰胆管注射5%牛磺胆酸钠法制备急性胰腺炎大鼠模型。全自动生化分析仪检测血清淀粉酶、脂肪酶水平,ELISA法检测血清TNF-α、IL-1β水平,HE染色观察大鼠胰腺和回肠组织病理变化,TUNEL染色法观察大鼠回肠组织细胞凋亡;实时荧光定量PCR(RT-q PCR)检测回肠组织mi R-320表达,蛋白质印迹法检测回肠组织JAK2/STAT3和NF-κB信号通路相关蛋白表达。结果:与假手术组相比,模型组大鼠胰腺和回肠损伤严重,病理学评分和回肠组织细胞凋亡率明显增加(P<0.05),血清淀粉酶、脂肪酶、TNF-α、IL-1β水平明显升高(P<0.05),回肠组织mi R-320表达明显降低(P<0.05),回肠组织p-JAK2/JAK2、p-STAT3/STAT3、p-p65/p65、p-IκBα/IκBα比值明显升高(P<0.05);与模型组相比,agomir mi R-320组、AG490组、PDTC组大鼠胰腺和回肠病理损伤减轻,病理学评分和回肠组织细胞凋亡率明显降低(P<0.05),血清淀粉酶、脂肪酶、TNF-α、IL-1β水平明显降低(P<0.05),回肠组织mi R-320表达明显升高(P<0.05),回肠组织p-JAK2/JAK2、p-STAT3/STAT3、p-p65/p65、p-IκBα/IκBα比值明显降低(P<0.05)。结论:Mi R-320可通过抑制JAK2/STAT3和NF-κB信号通路的激活改善急性胰腺炎大鼠肠道损伤。Background:Expression of microRNA⁃320(miR⁃320)is down regulated in acute pancreatitis,and the mechanism of its effect on acute pancreatitis is still unclear.Aims:To investigate the effect of miR⁃320 on intestinal injury in rats with acute pancreatitis and its mechanism.Methods:Rats were randomly divided into sham operation group,model group,miR⁃320 agonist group(agomir miR⁃320 group),miR⁃320 agonist control group(agomir NC group),JAK2 inhibitor group(AG490 group),and NF⁃κB pathway inhibitor group(PDTC group).The rat model of acute pancreatitis was established by retrograde injection of 5%sodium taurocholate to the bile duct.The automatic biochemical analyzer was used to detect serum levels of amylase and lipase;ELISA assay was used to detect serum levels of TNF⁃αand IL⁃1β;HE staining was used to observe the pathological changes of rat pancreas and ileum;TUNEL staining was used to observe cell apoptosis in rat ileum;real⁃time fluorescent quantitative PCR(RT⁃qPCR)was used to detect the expression of miR⁃320 in ileum tissue;Western blotting method was used to detect the expressions of JAK2/STAT3 and NF⁃κB signaling pathway related proteins in ileum.Results:Compared with sham operation group,the pancreas and ileum were severely injured in model group,and the pathological score and ileum cell apoptosis were significantly increased(P<0.05),serum levels of amylase,lipase,TNF⁃α,and IL⁃1βwere significantly increased(P<0.05),the expression of miR⁃320 in ileum tissue was significantly decreased(P<0.05),the ratios of p⁃JAK2/JAK2,p⁃STAT3/STAT3,p⁃p65/p65,and p⁃IκBα/IκBαin ileum tissue were significantly increased(P<0.05).Compared with model group,the pathological damages of pancreas and ileum in agomir miR⁃320 group,AG490 group and PDTC group were reduced,and the pathological score and ileum cell apoptosis were significantly decreased(P<0.05),serum levels of amylase,lipase,TNF⁃α,and IL⁃1βwere significantly decreased(P<0.05),the expression of miR⁃320 in ileum tissue wa

关 键 词：微RNA-320 急性胰腺炎 JAK2/STAT3 NF-κB 肠道损伤 

分 类 号：R576[医药卫生—消化系统]