枫蓼肠胃康联合5⁃FU抑制IL⁃6/STAT3通路治疗结肠炎相关性结肠癌的实验研究  

Study on the mechanism of action of Feng⁃Liao⁃Chang⁃Wei⁃Kang combined with 5⁃fluorouracil in the treatment of colitis⁃associated colon cancer

作　　者：汪倩如 钟立璠 黄凌 WANG Qian-ru;ZHONG Li-fan;HUANG Ling(Hainan Province Key Laboratory for Drug Preclinical Study of Pharmacology and Toxicology Research,Haikou 570100,China;Key Laboratory of Hainan Trauma and Disaster Rescue,Haikou 570100,China;Hainan Research Center for Drug Safety Evaluation,Hainan Medical College,Haikou 57119,China;School of Pharmaceutical Sciences Hainan Medical University,Haikou 57119,China)

机构地区：[1]海南省药物临床前药理毒理学研究重点实验室,海南海口570100 [2]海南省创伤与灾难救援研究重点实验室,海南海口570100 [3]海南医学院海南省药物安全性评价研究中心,海南海口571199 [4]海南医学院药学院,海南海口571199

出　　处：《海南医学院学报》2023年第18期1361-1366,共6页Journal of Hainan Medical University

基　　金：国家自然科学基金(81760674);海南省研究生创新科研课题(Hys2020⁃371)。

摘　　要：目的:探讨枫蓼肠胃康联合5⁃氟尿嘧啶给药对结肠炎相关性结肠癌小鼠的治疗作用及机制。方法:建立结肠炎相关性结肠癌小鼠模型,经枫蓼肠胃康和5⁃氟尿嘧啶给药后,观察小鼠的行为及活动情况;HE染色观察小鼠结肠组织的病理学变化;采用Western blot检测小鼠结肠组织在IL⁃6/STAT3通路相关蛋白的表达。结果:联合给药组小鼠的存活率明显提高,且小鼠肠壁增厚及间质炎症情况明显减轻。Western blot结果显示经造模后小鼠结肠组织中P⁃STAT3及IL⁃6的表达水平明显升高,联合给药后,抑制了IL⁃6/STAT3通路中Cyclin D1、CDK4和Bcl⁃2蛋白表达,上调了Bax的表达(P<0.05)。结论:枫蓼肠胃康联合5⁃氟尿嘧啶可抑制IL⁃6/STAT3通路来发挥对结肠炎相关性结肠癌的抑制作用。Objective:To investigate the therapeutic effects and mechanisms of Feng⁃Liao⁃Chang⁃Wei⁃Kang combined with 5⁃fluorouracil administration in mice with colitis⁃associated colon cancer.Methods:To establish a colitis⁃associated colon can⁃cer mouse model and observe the behavior and activity of mice after Feng⁃Liao⁃Chang⁃Wei⁃Kang and 5⁃fluorouracil administra⁃tion;HE staining was used to observe the pathological changes of mouse colonic tissue;Western blot was used to detect the ex⁃pression of mouse colon tissue in IL⁃6/STAT3 pathway⁃related proteins.Results:The survival rate of mice in the co⁃administered group was significantly increased,and the intestinal wall thickening and interstitial inflammation of mice were significantly reduced.Western blot results showed that the expression levels of P⁃STAT3 and IL⁃6 were significantly increased in the colonic tissue of mice after modeling,and the combined administration inhibited the expression of Cyclin D1,CDK4 and Bcl⁃2 protein in the IL⁃6/STAT3 pathway and upregulated the expression of Bax(P<0.05).Conclusion:Feng⁃Liao⁃Chang⁃Wei⁃Kang combined with 5⁃flu⁃orouracil inhibits IL⁃6/STAT3 pathway to exert inhibition of colitis⁃associated colon cancer inhibition of colitis⁃associated colon cancer.

关 键 词：结肠炎相关性结肠癌 枫蓼肠胃康 5⁃FU IL⁃6/STAT3 

分 类 号：R574.62[医药卫生—消化系统] R735.35[医药卫生—内科学]