小鼠未定带至PAG腹外侧区特异性神经元通路的形态学研究  

Morphological study of the specific neuronal pathways from zona incerta to target neurons of the ventrolateral periaqueductal gray in mice

作　　者：吕培源 冯慧洁 张蓬鑫 彭霏 薛懿珈 阮彩莲[3] 董玉琳 LYU Peiyuan;FENG Huijie;ZHANG Pengxin;PENG Fei;XUE Yijia;RUAN Cailian;DONG Yulin(Department of Anatomy,Histology and Embryology,K.K.Leung Brain Research Centre,Air Force Medical University,Xi’an 710032;The 4th Battalion,School of Basic Medicine,Air Force Medical University,Xi’an 710032;Department of Anatomy,School of Basic Medicine,Yan’an University,Yan’an 716000;The 5^(th)Battalion,School of Basic Medicine,Brigade of Basic Medical College,Air Force Medical University,Xi’an 710032,China)

机构地区：[1]空军军医大学基础医学院人体解剖学教研室暨梁銶琚脑研究中心,西安710032 [2]空军军医大学基础医学院学员四大队,西安710032 [3]延安大学基础医学院人体解剖学教研室,延安716000 [4]空军军医大学基础医学院学员五大队,西安710032

出　　处：《神经解剖学杂志》2023年第4期401-406,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(32271045);陕西省重点研发计划(2023⁃YBSF⁃156)。

摘　　要：目的:利用囊泡膜谷氨酸转运体2(VGluT2)⁃ires⁃cre、谷氨酸脱羧酶2(GAD2)⁃ires⁃cre和羟色胺转运体(Sert)⁃ires⁃cre小鼠结合重组狂犬病毒介导的逆行跨单级突触追踪技术,研究未定带(ZI)和中脑导水管周围灰质腹外侧区(vlPAG)内特异性神经元之间的纤维联系。方法:首先将辅助病毒注入转基因小鼠右侧vlPAG内,两周过后将重组狂犬病毒(RV)注入相同区域。一周后,在激光共聚焦显微镜下观察RV标记的突触前神经元在ZI内的分布。结果:将RV注入vlPAG后,在VGluT2⁃ires⁃cre小鼠ZI的吻尾方向上均可观察到逆标的突触前神经元分布;GAD2⁃ires⁃cre小鼠逆标神经元只见于ZI吻侧部,但Sert⁃ires⁃cre小鼠ZI内少见RV逆行标记的突触前神经元。结论:ZI神经元发出投射至vlPAG,作用于其中的谷氨酸能神经元或GABA能神经元,该通路可能参与了疼痛或恐惧样行为的调控。Objective:Using vesicular glutamate transporter(VGluT2)⁃ires⁃cre,glutamate decarboxylase 2(GAD2)⁃ires⁃cre,and serotonin transporter(Sert)⁃ires⁃cre mice combined with recombinational rabies virus(RV)⁃mediated retrogradely trans monosynaptic tracing system to observe the neuronal connections between zona incerta(ZI)and ventrolateral periaqueductal gray(vlPAG).Methods:The helper virus was first injected into the right vlPAG of transgenic mice,and RV was then injected into the same area two weeks later.One week later,the distributions of RV⁃labeled presynaptic neurons in ZI were observed under laser confocal microscope.Results:After RV injection into the vlPAG of VGluT2⁃ires⁃cre mouse ZI,the presynaptic neurons could be observed in the ZI from the rostral to caudal lev⁃el.The RV retrograde labeled neurons were found only in the rostral level of the ZI in the GAD2⁃ires⁃cre mouse.However,presynaptic neurons in the Sert⁃ires⁃cre mouse were rare.Conclusion:ZI sends projections to the vlPAG and mainly acts on the glutaminergic or GABAergic neurons,which may be involved in the regulation of nociception or fear⁃like behavior.

关 键 词：未定带 导水管周围灰质腹外侧区 逆行跨单级突触病毒 囊泡膜谷氨酸转运体2 γ⁃氨基丁酸 5⁃羟色胺 小鼠 

分 类 号：R74[医药卫生—神经病学与精神病学]