八肽胆囊收缩素在吗啡依赖及戒断大鼠相关脑区的表达变化  

Expression of cholecystokinin octapeptide in associated brain regions of morphine dependence and withdrawal rats

作　　者：于海磊 刘振江 文迪 李英敏 温伯扬 毕海涛 马春玲 YU Hailei;LIU Zhengjiang;WEN Di;LI Yingmin;WEN Boyang;BI Haitao;MA Chunling(College of Forensic Medicine,Hebei Key Laboratory of Forensic Medicine,Collaborative Innovation Center of Forensic Medial Molecular Identification,Hebei Medical University,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学法医学院,河北省法医学重点实验室,河北省法医分子鉴定协同创新中心,石家庄050017

出　　处：《神经解剖学杂志》2023年第4期413-419,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81273337,81772019);河北省高等学校科学技术研究项目(QN2015048)。

摘　　要：目的:探究慢性吗啡依赖及戒断大鼠相关脑区八肽胆囊收缩素(CCK⁃8)表达的变化。方法:利用Wistar大鼠建立慢性吗啡依赖及戒断模型,分为对照组(control)、吗啡依赖组(MOR)、纳洛酮催促戒断组(NAL)。Gellert⁃Holtzman评分评价吗啡成瘾及戒断程度,采用免疫组织化学和原位杂交技术分别检测相关脑区CCK⁃8蛋白及CCK mRNA表达的变化。结果:戒断组Gellert⁃Holtzman评分与对照组和吗啡依赖组比较显著增加;前额叶皮质(PFC)、黑质致密部(SNc)、中脑腹侧被盖区(VTA)CCK⁃8及CCK mRNA表达依赖组较对组明显升高,戒断后表达下降;杏仁核、蓝斑(LC)吗啡依赖后CCK⁃8及CCK mRNA表达较对照组显著增高,海马齿状回(PoDG)、杏仁核、LC戒断后较依赖组表达进一步升高;尾壳核(CPU)、伏隔核(NAc)、中脑导水管周围灰质(PAG)未检测到CCK⁃8及CCK mRNA的表达。结论:CCK⁃8参与了大鼠吗啡依赖与戒断过程并具有脑区特异性。Objective:To investigate the expression of cholecystokinin octapeptide(CCK⁃8)in associated brain region during morphine dependence and withdrawal status of rats.Methods:Wistar rats were randomly divided into control group,morphine induced dependence group and morphine withdrawal group.Gellert⁃holtzman score was used to evaluate the degree of morphine addiction and withdrawal.The expression of CCK⁃8 and its mRNA was detected by immunohistochemical staining(IHC)and hybridization in situ(ISH).Results:The Gellert⁃Holtzman score of the ab⁃stinence group were significantly higher than that of the control group and the morphine dependent group;IHC and ISH showed that the expression of CCK⁃8 in prefrontal cortex(PFC),substantia nigra pars compacta(SNc)and ventral teg⁃mental area(VTA)in dependent group was significantly higher than the control group but decreased during withdrawal status.The expression level of CCK⁃8 protein and mRNA of rats in polymorph layer of the dentate gyrus(PoDG),amygdaloid nucleus and locus ceruleus(LC)increased step by from morphine dependence status to abstinence state,and there were significant differences;CCK⁃8 were not detected by IHC and ISH in nulceus accumbens(NAc),cau⁃date putamen(CPU)and periaqueductal gray(PAG)in each group.Conclusion:CCK⁃8 is involved in the process of morphine dependence and withdrawal and has brain region specificity in rats.

关 键 词：吗啡依赖与戒断 八肽胆囊收缩素 原位杂交 脑区特异性 大鼠 

分 类 号：R749.6[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]