锆等离子体浸没离子注入增强聚醚醚酮材料的细胞相容性  

Zirconium with plasma immersion ion implantation enhances the cytocompatibility of polyether ether ketone materials

作　　者：许朗 黄忠卫 石锐[3] XU Lang;HUANG Zhong-wei;SHI Rui(Department of Stomatology,Beijing Jishuitan Hospital,Beijing 100035,China;Department of Stomatology,Beijing Royal Hospital of Integrated Traditional Chinese and Western Medicine,Beijing 102200,China;Beijing Research Institute of Traumatology and Orthopaedics/Research Institute of Beijing Jishuitan Hospital,Beijing 100035,China)

机构地区：[1]北京积水潭医院口腔科,北京100035 [2]北京王府中西医结合医院口腔科,北京102200 [3]北京市创伤骨科研究所/北京积水潭医院研究所,北京100035

出　　处：《局解手术学杂志》2023年第9期760-763,共4页Journal of Regional Anatomy and Operative Surgery

基　　金：国家自然科学基金面上项目(82072406)。

摘　　要：目的使用等离子体浸没离子注入技术(PIII)将锆离子注入到聚醚醚酮(PEEK)材料中,并初步评价该材料与人牙龈成纤维细胞(HGFs)的生物相容性。方法将经过PIII改性的PEEK试件作为改性组,未经改性的试件作为对照组,然后与HGFs在体外共培养,扫描电镜观察试件表面形貌,显微镜下对HGFs的细胞形态进行分析,CCK-8检测细胞增殖率以评价材料的生物相容性。结果PIII注入锆离子可在PEEK材料表面成功构建改性层。在培养4 h后,2组试件表面上均有HGFs铺展,且改性组试件表面细胞铺展更开,细胞形态更长,出现的伪足更多。在培养3 d、5 d、7 d后,改性组表面细胞吸光度值较对照组增高,差异有统计学差异(P<0.05)。结论锆等离子体浸没离子注入可在PEEK材料表面成功改性,材料表面的氧化锆纳米结构能够促进HGFs的黏附、增殖,有利于软组织黏附。Objective Zirconium ions were implanted into the polyether ether ketone(PEEK)materials by plasma immersion ion implantation(PIII),to preliminarily evaluatethe cytocompatibility of this material with human gingival fibroblasts(HGFs).Methods The PEEK specimens modified by PIII were used as the modification group,and the unmodified specimens were used as the control group,and then co-cultured with HGFs in vitro.The surface morphology was observed by scanning electron microscopy,the cell morphology was analyzed under a microscope,and the cell proliferation rate was determined by CCK-8 to evaluate the biocompatibility of the material.Results Zirconium ions with PIII implantation could successfully construct a modified layer on the surface of PEEK materials.After cultured for 4 hours,the surface of both groups of specimens had HGFs spreading,and the cells in the modified group were spread better,with longer cell morphology and more pseudopodia.After cultured for 3 days,5 days and 7 days,the cell absorbance values of surface in the modified group were higher than those in the control group,with statistically significant differences(P<0.05).Conclusion The surface of PEEK materials can be successfully modified with zirconium implantation by PIII,and the zirconia nanostructure on the surface of the material can promote the adhesion and proliferation of HGFs,which is conducive to soft tissue adhesion.

关 键 词：人牙龈成纤维细胞 聚醚醚酮 黏附 增殖 等离子体浸没离子注入技术 锆离子 

分 类 号：R783.1[医药卫生—口腔医学]