Circular RNA expression and the competitive endogenous RNA network in pathological,age-related macular degeneration events:A cross-platform normalization study  

作　　者：Ruxu Sun Hongjing Zhu Ying Wang Jianan Wang Chao Jiang Qiuchen Cao Yeran Zhang Yichen Zhang Songtao Yuan Qinghuai Liu 

机构地区：[1]Department of Ophthalmology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Medical University,Nanjing,Jiangsu 211166,China

出　　处：《The Journal of Biomedical Research》2023年第5期367-381,共15页生物医学研究杂志（英文版）

基　　金：funded by the National Natural Science Foundation of China(Grant No.81970821);the Postgraduate Research Innovation Program of Jiangsu Provinc(Grant No.SJCX21_0624).

摘　　要：Age-related macular degeneration(AMD)causes irreversible blindness in people aged over 50 worldwide.The dysfunction of the retinal pigment epithelium is the primary cause of atrophic AMD.In the current study,we used the ComBat and Training Distribution Matching method to integrate data obtained from the Gene Expression Omnibus database.We analyzed the integrated sequencing data by the Gene Set Enrichment Analysis.Peroxisome and tumor necrosis factor-α(TNF-α)signaling and nuclear factor kappa B(NF-κB)were among the top 10 pathways,and thus we selected them to construct AMD cell models to identify differentially expressed circular RNAs(circRNAs).We then constructed a competing endogenous RNA network,which is related to differentially expressed circRNAs.This network included seven circRNAs,15 microRNAs,and 82 mRNAs.The Kyoto Encyclopedia of Genes and Genomes analysis of mRNAs in this network showed that the hypoxia-inducible factor-1(HIF-1)signaling pathway was a common downstream event.The results of the current study may provide insights into the pathological processes of atrophic AMD.

关 键 词：age-related macular degeneration retinal pigment epithelium circular RNA bioinformatics analysis competing endogenous RNA 

分 类 号：R774.5[医药卫生—眼科]