裂分适配体传感器在小分子检测领域中的应用  

Application of split aptamers-based sensors for the detection of small molecules

作　　者：姜岩[1] 孙岐峰 杨咏洁[1] JIANG Yan;SUN Qifeng;YANG Yongjie(Department of Food and Biological Sciences College,Agriculture College of Y anbian University,Yanji 133002,China;Bejjing Hightrust Medical Laboratory Co.,Ltd,Beijing 102600,China)

机构地区：[1]延边大学农学院食品与生物科学系,延吉133002 [2]北京海思特医学检验实验室有限公司,北京102600

出　　处：《分析试验室》2023年第8期1116-1126,共11页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(31602126);吉林省教育厅基金(JJKH20180901KJ);延边大学青年基金(602017020);延边大学应用基础研究项目(602021007)资助。

摘　　要：裂分适配体(SPA)传感器是由2条或以上的寡核苷酸序列组装而成的一种新型生物传感装置。该装置必须在特异性靶标诱导下才能被成功组装,可以避免假阳性结果,故具有高度的特异性和灵敏度。SPA适用于各类型靶标的检测,尤其是小分子靶标,展现了极大的优势和潜力。然而,SPA传感器的开发及应用也极具挑战性,特别是如何改善SPA-靶标复合体的稳定性是目前主要难点。本文对SPA、裂分策略及其应用于小分子检测的研究现状进行了综述,以期为开发新型灵敏且特异的适配体传感器提供思路。The split aptamers(SPAs)-based sensors is a novel kind of biosensors,assembled by two or more oligonucleotides in the presence of specific targets.To be successfully assembled,the sensor has to be induced by a specific target,which can aviod false-positive results and thus has a high degree of specificity and sensitivity.SPAs are suitable for the detection of various targets and show great advantages and potential in the development of aptasensors,especially for the detection of small molecules.However,the development and application of SPA-based sensors still remain challenging.Currently,the major difficulty is how to improve the stability of SPA-target complexes.Herein,this review summarizes the SPAs,strategies of spliting aptamers,and their applications in the detection of small molecules,aiming to provide new ideas for the development of novel,sensitive,and specific aptasensors.

关 键 词：裂分适配体 裂分策略 小分子化合物 生物传感器 

分 类 号：TS201.6[轻工技术与工程—食品科学]