不同观察时间对幽门螺杆菌快速尿素酶试验诊断准确性的研究  

Study on diagnostic accuracy of Helicobacter pylori rapid urease test with different observation time

作　　者：石小琼[1] 殷国建[2] Shi Xiaoqiong;Yin Guojian(Department of Gastroenterology,Sichuan Science City Hospital,Mianyang 621000,China;Department of Gastroenterology,The Second Affiliated Hospital of Soochow University,Suzhou 215000,China)

机构地区：[1]四川省科学城医院消化内科,四川绵阳621000 [2]苏州大学附属第二医院消化内科,江苏省215000

出　　处：《中华消化病与影像杂志（电子版）》2023年第3期150-154,共5页Chinese Journal of Digestion and Medical Imageology(Electronic Edition)

摘　　要：目的观察不同时间段幽门螺杆菌(Hp)快速尿素酶试验(RUT)的准确性,为今后临床、科研提供参考数据。方法选择2022年1-12月于四川省科学城医院就诊,2周内未服用质子泵抑制剂、4周内未服用抗生素且需要胃镜检查的患者共58例,均行RUT、^(13)C尿素呼气试验及内镜下Hp诊断,以^(13)C尿素呼气试验和内镜下Hp诊断检测结果一致时作为金标准,比较RUT中延长观察时间对其诊断的准确性,并观察RUT检测最适观察时间。结果58例患者中Hp阳性32例,Hp阴性26例。RUT检测观察1、3、6、9、12、15 min的32例Hp阳性准确率分别为75%、87.5%、93.7%、100%、100%、100%;26例Hp阴性准确率分别为53.85%、69.23%、80.77%、92.31%、100%、100%。1、3 min考虑假阳性或假阴性的患者,将不同时间RUT检测试纸图片给不同职称医生进行盲法判断,显示同一时间点医师之间判断结果差异无统计学意义(P>0.05)。结论RUT检测中延长观察时间可增加其准确性。RUT检测中最适观察时间为9~12 min,可减少假阳性及假阴性的发生率。RUT检测结果的影响与医师无明显相关性。Objective To observe the diagnostic accuracy of Helicobacter pylori(Hp)rapid urease test(RUT)for different observation time,so as to provide reference for clinical and scientific research in the future.Methods A total of 58 patients who were admitted to Sichuan Science City Hospital from January to December 2022 and did not take proton pump inhibitors within 2 weeks and antibiotics within 4 weeks and required gastroscopy were selected.They all participated RUT,^(13)C urea breath test and endoscopic Hp diagnosis.When the results of^(13)C urea breath test and endoscopic Hp diagnostic test were consistent,the consistent result served as the gold standard.The diagnostic accuracies of RUT in different observation time were compared and the optimal observation time of RUT was observed.Results A total of 58 patients were included,including 32 Hp positive cases and 26 Hp negative cases.The positive accuracies of 32 Hp positive cases accepted RUT were 75%,87.5%,93.7%,100%,100%and 100%in 1 minute,3 minutes,6 minutes,9 minutes,12 minutes and 15 minutes,respectively.The accuracies of 26 cases of Hp negative cases were 53.85%,69.23%,80.77%,92.31%,100%and 100%,respectively.For patients with false positives or false negatives in 1 minute and 3 minutes,the RUT dipstick pictures of different times were tested for blind judgment by doctors with different professional titles,and the results showed no significant difference between doctors at the same time point(P>0.05).Conclusion Prolonging the observation time can increase the diagnostic accuracy of RUT,and the optimal observation time of RUT test is 9-12 minutes,which can reduce the incidence of both false positive and false negative results.The accuracy of RUT is not affected by the professional title of the detected doctors.

关 键 词：幽门螺杆菌 快速尿素酶试验 ^(13)C呼气试验 

分 类 号：R573[医药卫生—消化系统]