砷胁迫下石油烃降解菌的分离、鉴定及其降解特性  

ISOLATION, IDENTIFICATION AND DEGRADATION CHARACTERISTICS OF STRAINS FORREMEDIATION OF PETROLEUM HYDROCARBON UNDER ARSENIC STRESS

作　　者：李虹呈 苏趋 张武竹 张耀 向罗京 LI Hongcheng;SU Qu;ZHANG Wuzhu;ZHANG Yao;XIANG Luojing(Hubei Academy of Environmental Science,Wuhan 430072,China;State Key Laboratory of Soil Health Diagnosis and Green Remediation for Environmental Protection,Wuhan 430072,China;College of Resources and Environment,Huazhong Agricultural University,Wuhan 430070,China)

机构地区：[1]湖北省生态环境科学研究院(省生态环境工程评估中心),武汉430072 [2]国家环境保护土壤健康诊断与绿色修复重点实验室,武汉430072 [3]华中农业大学资源与环境学院,武汉430070

出　　处：《环境工程》2023年第7期166-174,共9页Environmental Engineering

基　　金：国家重点研发计划项目(2019YFC1804003);湖北省省级环保科研项目(2020年)。

摘　　要：以湖北省某砷-石油烃复合污染场地的土壤为研究对象,分离纯化砷胁迫下石油烃降解菌,采用16S rDNA测序技术进行菌种鉴定,分析降解菌的生长特性与降解特征,验证降解菌对复合污染土壤的实际修复效果。结果表明:从耐As高效石油烃降解菌株系列中筛选出菌株JYZ-03,其鉴定结果为不动杆菌(Acinetobacter sp.);菌株JYZ-03最佳生长和降解条件为pH=7、温度30℃、盐度0.1%和初始接种量2%;此条件下菌株JYZ-03对石油烃的降解效率为84.05%,对石油烃各组分降解能力存在差异,难易程度表现为长链烷(C26—C38)>多环芳烃>支链烷烃>中长碳链烷烃(C11—C25),石油烃降解效果显著,具有较好的实际修复效果。该研究丰富了石油烃污染修复功能菌株库,可为复合污染场地修复提供更多选择。In this study,petroleum hydrocarbon-degrading strains were isolated and purified from an arsenic-petroleum hydrocarbon composite contaminated site in Hubei Province.16S rDNA sequencing technology was used to identify the bacteria species.The growth and degradation characteristics of the degrading strain were analyzed,and the actual remediation ability of the degrading strains was tested on the composite contaminated soil.The main conclusions were as follows:Acinetobacter sp.JYZ-03,a highly efficient petroleum hydrocarbon degradation strain resistant to As,was isolated.The optimal growth and degradation conditions were pH of 7,30℃,salinity of 0.1%and initial inoculation amount of 2%.The degradation rate of petroleum hydrocarbon by strain JYZ-03 was 84.05%.The degradation ability of strain for different petroleum hydrocarbon components was in sequence of long-chain alkane(C26 to C38)>polycyclic aromatic hydrocarbons>branched alkanes>medium and long-chain alkanes(C11 to C25);the actual repairing by the strain achieved a satisfying effect.This study enriches the functional strains for remediation of petroleum hydrocarbon,and provides more options for the remediation of compoundcontaminated sites.

关 键 词：石油烃 砷 降解菌 土壤复合污染 生物修复 

分 类 号：X172[环境科学与工程—环境科学] X53