延边黄牛臀肉与眼肉组织非靶向代谢组学比较分析  

Comparative Analysis of Non-targeted Metabolomics between Rump and Eye Tissues of Yanbian Yellow Cattle

作　　者：孙斌 崔岩 王伟利 唐琳 王英[1] 李强[1] 李香子[1] SUN Bin;CUI Yan;WANG Weili;TANG Lin;WANG Ying;LI Qiang;LI Xiangzi(Northeast Cold Region Beef Cattle Technology Innovation Engineering Research Center of the Minis-try of Education/Jilin Province Beef Cattle Science and Industrial Technology Major Demand Collab-orative Innovation Center,Yanbian University,Yanji 133002,China;Changchun Customs Technol-ogy Center,Changchun 130062,China)

机构地区：[1]延边大学东北寒区肉牛科技创新教育部工程研究中心/吉林省肉牛科学与产业技术重大需求协同创新中心,延吉133002 [2]长春海关技术中心,长春130062

出　　处：《吉林农业大学学报》2023年第4期451-460,共10页Journal of Jilin Agricultural University

基　　金：国家自然科学基金项目(32060767);东北寒区肉牛科技创新教育部工程研究中心及高等学校学科创新引智计划项目(D20034)。

摘　　要：从非靶向代谢组学角度分析延边黄牛臀肉与眼肉组织代谢物谱的差异。试验以6头同月龄、同生长条件的延边黄牛屠宰后的臀肉和眼肉为试验材料,利用气相色谱-质谱联用仪(GC-MS)对代谢物进行分离,利用主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)等统计方式对数据进行分析。结果表明:试验筛选出差异代谢通路,分别为苯丙氨酸代谢通路,乙醛酸和二羧酸代谢通路,磷酸戊糖途径通路,甘氨酸、丝氨酸和苏氨酸代谢通路,精氨酸和脯氨酸代谢通路,嘌呤代谢通路。臀肉组与眼肉组的差异代谢物单变量统计分析(UVA),筛选出差异代谢物20种,其中11种差异代谢物发生上调(P<0.05),9种差异代谢物发生下调(P<0.05)。综上,非靶向代谢组学分析揭示,臀肉与眼肉在代谢水平上存在显著差异,油酸等差异代谢物将作为风味化合物的前体物质进而影响风味。The experiment aims to analyze the differences in metabolite profiles between rump and eye tissues of Yanbian yellow cattle from non-targeted metabolomics.The experiment used the rump and eye tissues of 6 Yanbian yellow cattle of the same month age and growth conditions as experimental materials.The metabolites were separated by GC-MS,and the data were analyzed by statistical methods such as PCA,OPLS-DA and UVA.The results were as follows:The experiment screened out differential metabolic pathways,including phenylalanine metabolism,glyoxylic and dicarboxylic acid metabolism,pentose phosphate pathway,glycine,serine and threonine metabolism,arginine and proline metabolism,and purine metabolism.The results of the screening of differential metabolites in rump group and eye group were as follows:20 different metabolites were screened out,of which 11 were upregulated(P<0.05),and 9 were downregulated(P<0.05).In summary,non-targeted metabolomics analysis reveals significant differences in metabolic levels between rump meat and eye meat.Different metabolites such as oleic acid will serve as precursors of flavor compounds and thus affect flavor.

关 键 词：延边黄牛 臀肉 眼肉 代谢组学 差异代谢通路 

分 类 号：S823.81[农业科学—畜牧学]