Curcumin alleviated dextran sulfate sodium-induced colitis by recovering memory Th/Tfh subset balance  

作　　者：Lin-Xin Zheng Kai-En Guo Jia-Qi Huang Miao-Hua Liu Bai-Ling Deng Duan-Yong Liu Bu-Gao Zhou Wen Zhou You-Bao Zhong Hai-Mei Zhao 

机构地区：[1]Department of Postgraduate,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [2]College of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [3]Formula-Pattern Research Center,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China [4]Nanchang Medical College,Nanchang 330052,Jiangxi Province,China [5]Laboratory Animal Research Center for Science and Technology,Jiangxi University of Chinese Medicine,Nanchang 330004,Jiangxi Province,China

出　　处：《World Journal of Gastroenterology》2023年第36期5226-5239,共14页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.81760808 and No.82260863;Scientific and Technological Project of Education Department of Jiangxi Province,No.GJJ181582;Jiangxi University of Chinese Medicine Science and Technology Innovation Team Development Program,No.CXTD22008;Key Laboratory of Pharmacodynamics and Quality Evaluation on anti-Inflammatory Chinese Herbs,Jiangxi Administration of Traditional Chinese Medicine,No.202208.

摘　　要：BACKGROUND Restoration of immune homeostasis by targeting the balance between memory T helper(mTh)cells and memory follicular T helper(mTfh)cells is a potential therapeutic strategy against ulcerative colitis(UC).Because of its anti-inflammatory and immunomodulatory properties,curcumin(Cur)is a promising drug for UC treatment.However,fewer studies have demonstrated whether Cur can modulate the mTh/mTfh subset balance in mice with colitis.AIM To explore the potential mechanism underlying Cur-mediated alleviation of colitis induced by dextran sulfate sodium(DSS)in mice by regulating the mTh and mTfh immune homeostasis.METHODS Balb/c mice were administered 3%and 2%DSS to establish the UC model and treated with Cur(200 mg/kg/d)by gavage on days 11-17.On the 18th d,all mice were anesthetized and euthanized,and the colonic length,colonic weight,and colonic weight index were evaluated.Histomorphological changes in the mouse colon were observed through hematoxylin-eosin staining.Levels of Th/mTh and Tfh/mTfh cell subsets in the spleen were detected through flow cytometry.Western blotting was performed to detect SOCS-1,SOCS-3,STAT3,p-STAT3,JAK1,p-JAK1,and NF-κB p65 protein expression levels in colon tissues.RESULTS Cur effectively mitigates DSS-induced colitis,facilitates the restoration of mouse weight and colonic length,and diminishes the colonic weight and colonic weight index.Simultaneously,it hinders ulcer development and inflammatory cell infiltration in the colonic mucous membrane.While the percentage of Th1,mTh1,Th7,mTh7,Th17,mTh17,Tfh1,mTfh1,Tfh7,mTfh7,Tfh17,and mTfh17 cells decreased after Cur treatment of the mice for 7 d,and the frequency of mTh10,Th10,mTfh10,and Tfh10 cells in the mouse spleen increased.Further studies revealed that Cur administration prominently decreased the SOCS-1,SOCS-3,STAT3,p-STAT3,JAK1,p-JAK1,and NF-κB p65 protein expression levels in the colon tissue.CONCLUSION Cur regulated the mTh/mTfh cell homeostasis to reduce DSS-induced colonic pathological damage,potentially by suppressing th

关 键 词：CURCUMIN Ulcerative colitis Memory T helper Memory follicular T helper JAK1/STAT3/SOCS 

分 类 号：R574.62[医药卫生—消化系统]