匙羹藤中一个新的C_(21)甾体糖苷及其降血糖活性  

A New C_(21)Steroidal Glycoside from Gymnema sylvestre and Its Hypoglycemic Activity

作　　者：刘美余 张进燕 周童曦 廖广凤 杨新洲[2] 卢汝梅 LIU Meiyu;ZHANG Jinyan;ZHOU Tongxi;LIAO Guangfeng;YANG Xinzhou;LU Rumei(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning Guangxi 530000,China;School of Pharmaceutical Sciences,South-Central University for Nationalities,Wuhan Hubei 430074,China)

机构地区：[1]广西中医药大学药学院,广西南宁530000 [2]中南民族大学药学院,湖北武汉430074

出　　处：《广西师范大学学报（自然科学版）》2023年第5期96-104,共9页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(81860750);广西研究生教育创新计划资助项目(YCSW2021218);广西中医药大学桂派中医药传承创新团队资助项目(2022B005)。

摘　　要：综合运用大孔树脂、硅胶、Sephadex LH-20柱色谱和半制备高效液相色谱等多种分离方法对壮药匙羹藤Gymnema sylvestre(Retz.)Schult.乙醇提取物的化学成分进行分离纯化,从中分离得到16个化合物,通过理化性质和波谱数据鉴定化合物结构,分别为sylvepregoside E(1)、gymnepregoside H(2)、芹菜素(3)、木犀草素(4)、对羟基苯甲醛(5)、对羟基苯甲酸(6)、桂皮酸(7)、咖啡酸(8)、3,4-二甲氧基肉桂酸(9)、反式对羟基肉桂酸乙酯(10)、4-O-β-D-吡喃葡萄糖基香草酸(11)、ochuscin C(12)、松脂醇(13)、4-羟基-2-壬烯酸(14)、亚麻酸(15)和亚油酸(16)。其中化合物1为新化合物,其余化合物均为首次从该植物中分得。生物活性实验结果表明,化合物1和2可显著促进L6细胞对葡萄糖的摄取,葡萄糖摄取量分别是空白组的1.79和1.95倍,具有较强的降血糖活性。The chemical constituents of Gymnema sylvestre(Retz.)Schult.were isolated and purified by macroporous adsorption resin,silica gel,Sephadex LH-20 and semipreparative high performance liquid chromatography.Their structures were identified by physicochemical properties and spectral data.Sixteen compounds were isolated and identified as sylvepregoside E(1),gymnepregoside H(2),apigenin(3),luteolin(4),phydroxybenzaldehyde(5),phydroxybenzoic acid(6),cinnamic acid(7),caffeic acid(8),3,4-dimethoxycinnamic acid(9),transphydroxycinnamic acid ethyl ester(10),4-O-β-D-glucopyranosylvanillic acid(11),ochuscin C(12),pinoresinol(13),4-hydroxy-2-nonenoic acid(14),linolenic acid(15),and linoleic acid(16).Among them,compound 1 was a new compound,compounds 2-16 were isolated from this plant for the first time.The results of biological activity showed that compounds 1 and 2 could significantly promote the glucose uptake of L6 cells,which were 1.79 and 1.95 times higher than that of the blank group,respectively.

关 键 词：匙羹藤 C_(21)甾体糖苷 sylvepregoside E gymnepregoside H 降血糖 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]