A redox-responsive self-assembling COA-4-arm PEG prodrug nanosystem for dual drug delivery suppresses cancer metastasis and drug resistance by downregulating hsp90 expression  

作　　者：Yi Zhou Yingling Miao Qiudi Huang Wenwen Shi Jiacui Xie Jiachang Lin Pei Huang Chengfeng Yue Yuan Qin Xiyong Yu He Wang Linghao Qin Jianhai Chen 

机构地区：[1]Nanfang Hospital,Southern Medical University,Guangzhou 510515,China [2]Key Laboratory of Molecular Target and Clinical Pharmacology and the State Key Laboratory of Respiratory Disease and the Fifth Affiliated Hospital,School of Pharmaceutical Sciences,Guangzhou Medical University,Guangzhou 511436,China [3]Center of Cancer Research,The Second Affiliated Hospital,Guangzhou Medical University,Guangzhou 510260,China [4]School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China

出　　处：《Acta Pharmaceutica Sinica B》2023年第7期3153-3167,共15页药学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.32101105;81373339);the 2021 Natural Science Foundation of Guangdong Province(Nos.2021A1515011367,China);the Southern Hospital Matching Fund(Nos.2013001,China);the High-Level university Academic Backbone and Training program in Guangzhou Medical University(Nos.B185004199,China);2022 City school joint funding project(Nos.202201020394,China);the 2018 Guangdong Key Discipline Construction Project of Pharmacy(Nos.Q185031010,China);the 2019 Undergraduate Laboratory Open Project(Nos.C195015003,China);Guangzhou Science and Technology Planning Project(Nos.202201010783,China)。

摘　　要：Metastasis and resistance are main causes to affect the outcome of the current anticancer therapies.Heat shock protein 90(Hsp90)as an ATP-dependent molecular chaperone takes important role in the tumor metastasis and resistance.Targeting Hsp90 and downregulating its expression show promising in inhibiting tumor metastasis and resistance.In this study,a redox-responsive dual-drug nanocarrier was constructed for the effective delivery of a commonly used chemotherapeutic drug PTX,and a COAmodified 4-arm PEG polymer(4PSC)was synthesized.COA,an active component in oleanolic acid that exerts strong antitumor activity by downregulating Hsp90 expression,was used as a structural and functional element to endow 4PSC with redox responsiveness and Hsp90 inhibitory activity.Our results showed that 4PSC/PTX nanomicelles efficiently delivered PTX and COA to tumor locations without inducing systemic toxicity.By blocking the Hsp90 signaling pathway,4PSC significantly enhanced the antitumor effect of PTX,inhibiting tumor proliferation and invasiveness as well as chemotherapy-induced resistance in vitro.Remarkable results were further confirmed in vivo with two preclinical tumor models.These findings demonstrate that the COA-modified 4PSC drug delivery nanosystem provides a potential platform for enhancing the efficacy of chemotherapies.

关 键 词：3-O-(Z)-Coumaroyloleanolic acid Cancer metastasis Drug resistance HSP90 Codelivery Prodrug nanosystem Chemotherapies Redox responsiveness 

分 类 号：TB383.1[一般工业技术—材料科学与工程] R730.5[医药卫生—肿瘤]