High-throughput base editing KO screening of cellular factors for enhanced GBE  

作　　者：Jie Yang Dongdong Zhao Xiagu Zhu Yuanzhao Yang Bo Li Siwei Li Chang-Hao Bi Xue-Li Zhang 

机构地区：[1]College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300222,China [2]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China [3]Key Laboratory of Systems Microbial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China [4]National Technology Innovation Center of Synthetic Biology,Tianjin 300308,China [5]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Journal of Genetics and Genomics》2023年第8期611-614,共4页遗传学报（英文版）

基　　金：financially supported by the National Key Research and Development Program of China(2018YFA0901300);the National Natural Science Foundation of China(32171449,81903776);a Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-001);Tianjin Natural Science Foundation(20JCYBJC00310);Youth Innovation Promotion Association CAS(2022177).

摘　　要：Base editor techniques have been developed as a means of precisely converting bases without the need for double-stranded DNA breaks(DSBs)or editing templates.Currently,these techniques can be used for cytosine(C)to thymine(T)conversions(cytosine base editors,CBEs)(Komor et al.,2016;Nishida et al.,2016),adenine(A)to guanine(G)conversions(adenine base editors,ABEs)(Gaudelli et al.,2017),and cytosine(C)to guanine(G)conversions(glycosylase base editors,GBEs)(Zhao et al.,2021)in mammalian cells.GBE,in particular,is a promising base editing technique capable of correcting up to 11%of the 32,044 pathogenic single nucleotide polymorphisms(SNPs)known to date(Gaudelli et al.,2017).Despite its potential,the performance of GBE is still not optimal,and its editing outcomes exhibit a wider variation range than those of CBEs due to the dependence on cellular DNA repair systems(Jiang et al.,2021),which implies that efficient GBE performance remains a challenge.

关 键 词：GBE BASE CBE 

分 类 号：Q811.4[生物学—生物工程]