Reducing Yield of Fusel Alcohols by Saccharomyces cerevisiae of Compound Mutagenesis Through UV-MPMS Method  

作　　者：Liu Zi-wei Wang Xin-xiu Wu Si Chen Zuo-hui Zhang Hui Sun Yao Hui Mi-zhou Shuang Bao 

机构地区：[1]College of Life Sciences,Northeast Agricultural University,Harbin 150030,China

出　　处：《Journal of Northeast Agricultural University(English Edition)》2023年第3期85-96,共12页东北农业大学学报（英文版）

基　　金：Supported by Heilongjiang Natural Science Foundation Joint Guide Project(LH2019C022)。

摘　　要：Excessive fusel alcohol contents will cause the beer to produce off-flavors and cause dizziness and headaches.Reducing the contents of fusel alcohols in beer is very important to people's health.The excessive fusel alcohol contents in beer is a common problem in the industry.How to control the contents of fusel alcohols in a reasonable range is of great significance for improving beer quality.After one round of ultraviolet(UV)and one round of multifunctional plasma mutagenesis system(MPMS)mutagenesis,the yeast strains with lower fusel oil yield and more stablility could be screened.According to the relationship between the fusel alcohol Harris metabolic pathway of brewer's yeast and lactic acid metabolism,excellent strains were obtained by triple screening with lactic acid medium,calcium carbonate medium and 2,3,5-triphenyl tetrazolium chloride upper medium.The content of fusel alcohol in the finished beer fermentation test of screened strain Z43 was 52.1±0.142 mg•L^(-1),which was 43%lower than that of the starting strain,and other fermentation properties remained unchanged.After eight passages,it was verified that the strain was stable and heritable.These results showed that strain Z43 presented promising characteristics for use in the production of beer with a potentially low contents of fusel alcohols.

关 键 词：Saccharomyces cerevisiae fusel alcohol UV mutagenesis MPMS mutagenesis strain screening 

分 类 号：TS261.11[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]