微波辐射和浸种对华山松种子生活力和发芽的影响  

Effects of Microwave Radiation and Seed Soaking on Seed Viability and Germination of Pinus armandii

作　　者：张合瑶 马福仙 李莲芳 顾梦 刘娴 李小军 周冬梅 Zhang Heyao;Ma Fuxian;Li Lianfang;Gu Meng;Liu Xian;Li Xiaojun;Zhou Dongmei(College of Forestry,Southwest Forestry University,Kunming Yunnan 650233,China)

机构地区：[1]西南林业大学林学院,云南昆明650233

出　　处：《西南林业大学学报（自然科学）》2023年第5期23-30,共8页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家“十三五”重点研发计划项目(2017YED0600500)资助。

摘　　要：为了解微波辐射和清水2因素不同水平组合对华山松种子吸水、生活力和种仁发芽率的影响,以及微波辐射结合清水和GA_(3)溶液浸种3因素对华山松种子发芽率的影响,采用L^(9)(3^(4))正交设计开展试验实施。结果表明:微波辐射和清水浸种处理组合的种子生活力、种子、种仁和种壳吸水率分别为75.3%~88.0%(CK的为94.7%)、6.63%~10.44%、15.00%~23.80%和0.98%~4.87%,处理组合间种子和种仁吸水率均具有极显著的差异(P<0.01);影响生活力的主导因子是微波辐射和清水浸种时间的交互作用,影响种子和种仁吸水率的则是清水浸种时间;测定(TTC法)的和未测定生活力的种仁发芽率分别为4.0%~22.8%和52.7%~90.0%,CK的为4.7%和60.0%,微波辐射是影响种仁发芽率的主导因子。微波辐射结合清水和GA_(3)溶液浸种的组合,种子场圃发芽率为56.0%~71.6%(CK的为56.0%),清水和GA_(3)浸种的交互作用是影响种子场圃发芽率的主导因子。微波辐射和清水浸种的组合降低种子生活力。清水浸种8 h时,种子吸水基本达到饱和;微波辐射15 s,清水浸种12 h后0.05 g/L GA_(3)溶液浸种,提高华山松种子场圃发芽率,可应用于生产实践。In order to understand the effects of different levels with which treatment combinations(TCs)of the 2 factors of microwave radiation and clear water on water absorption and viability of seeds,involved germination rate of seed kernel.And the effects of microwave radiation combined with clear water and GA_(3)seed soaking on germination rate of seeds for Pinus armandii.The L^(9)(3^(4))orthogonal design was applied to implement the experiments.The results show that the seed viability,water absorption rates of seed,kernel and seed shell for the TCs of microwave radiation and water soaking were 75.3%-88.0%(94.7%of the control),6.63%-10.44%,15.00%-23.80%and 0.98%-4.87%,respectively,and with significant differences in seed and kernel water absorption between the TCs(P<0.01).The dominant factor affecting viability was the interaction between microwave radiation and water soaking time,and the water absorption of seeds and kernels was affected by the soaking time of seeds in clear water;the germination rates of seeds with and without viability measured(TTC method)were 4.0%-22.8%and 52.7%-90.0%,respectively,and those of the control were 4.7%and 60.0%.Microwave radi-ation was the dominant factor affecting the germination rate.Nursery germination rates of seeds radiated with mi-crowave combined seeds soaked with water and GA_(3)were 56.0%-71.6%(56.0%of the control),with the interac-tion between water and GA_(3)soaked seeds was the dominant factor affecting the germination rate.The combina-tion of microwave radiation and soaking seeds in water reduced seed viability.When the seeds were soaked in wa-ter for 8 hours,the water absorption of the seeds basically reached saturation.Soaking seeds in microwave radi-ation for 10 s,and clear water for 8 h after 0.05 g/L GA_(3)solution could improve the germination rate of P.ar-mandii seeds,which could be applied to production practice.

关 键 词：华山松 微波辐射 浸种 生活力 种子吸水 发芽率 

分 类 号：S791.241[农业科学—林木遗传育种]