基于实时荧光定量PCR技术的肿瘤分子病理检测临床实践中国专家共识(2023版)  

Chinese expert consensus on clinical practice of quantitative real-time polymerase chain reaction techniques in molecular pathological diagnostics of tumor(2023 edition)

作　　者：中国抗癌协会肿瘤病理专业委员会 中国抗癌协会肿瘤基因诊断专业委员会 中国研究型医院学会超微与分子病理学专业委员会 北京市病理质量控制和改进中心 李文斌 蒋兴然 魏冰 郭蕾[5] 董周寰[6] 李卫华 The Society of Tumor Pathology of Chinese Anti-Cancer Association;The Society of Gene Diagnosis of Tumor of Chinese Anti-Cancer Association;The Society of Ultrastructural and Molecular Pathology of Chinese Research Hospital Association;Beijing Quality Control and Quality Improvement Center of Pathology;Li Wenbin;Jiang Xingran;Wei Bing(不详;Department of Pathology,National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100021,China;Department of Pathology,Bejing Chao-Yang Hospital,Capital Medical University,Bejing 100020,China;Department of Molecular Pathology,Henan Key Laboratory of Molecular Pathology,the Afiliated Cancer Hospital of Zhengzhou University&Henan Cancer Hospital,Zhengzhou 450008,China)

机构地区：[1]不详 [2]国家癌症中心、国家肿瘤临床医学研究中心、中国医学科学院北京协和医学院肿瘤医院病理科,北京100021 [3]首都医科大学附属北京朝阳医院病理科,北京100020 [4]郑州大学附属肿瘤医院、河南省肿瘤医院临床病理中心分子病理科,郑州450008 [5]中国医学科学院肿瘤医院病理科 [6]解放军总医院第一医学中心病理科

出　　处：《中华肿瘤杂志》2023年第9期763-772,共10页Chinese Journal of Oncology

基　　金：中国癌症基金会北京希望马拉松专项基金(LC2022L02);中国医学科学院医学与健康科技创新工程(2021-I2M-1-066)。

摘　　要：基于实时荧光定量PCR(qPCR)技术的肿瘤分子病理检测具有操作简便、报告周期短、灵敏度高、结果判读标准化等优点。然而,在临床实践中,qPCR技术的性能验证、质量管理、异常结果处理等问题相对复杂,缺乏统一规范和标准。因此,专家共识旨在从qPCR检测全流程角度,对影响检测结果的重要环节、检测过程中遇到的常见问题及异常结果判定及处理等提供规范化意见,达成基于qPCR技术的肿瘤分子病理检测临床实践中国专家共识,以规范检测流程并提高结果准确性,促进技术发展和更为广泛的临床应用。Quantitative real-time polymerase chain reaction(qPCR)is one of the most widely used molecular pathological diagnostic techniques in China due to its advantages of the simple operation,short turnaround time,high sensitivity,and standardizable result analysis.However,in clinical practice,there is not yet an expert consensus to guide molecular pathological diagnostics of tumor using qPCR techniques in terms of validation and verification of method performance,quality management and interpretation of complex results.Therefore,this expert consensus aims to provide standardized opinions on the practical application of qPCR techniques,and suggestions on how to deal with common problems and abnormal results,and reach a Chinese expert consensus on the clinical practice of qPCR techniques in molecular pathological diagnostics of tumor,in order to standardize the testing process,improve the accuracy of results,and promote the clinical applications of qPCR techniques.

关 键 词：肿瘤 实时荧光定量PCR 分子检测 分子病理 专家共识 

分 类 号：R730.2[医药卫生—肿瘤]