慢性牙周炎与慢性阻塞性肺疾病的潜在共同分子机制及其转录因子初探  

Exploratory research on the probable shared molecular mechanism and transcription factors between chronic periodontitis and chronic obstructive pulmonary disease

作　　者：张晨[1] 候珍珍 宗颖睿 Zhang Chen;Hou Zhenzhen;Zong Yingrui(Dept.of Preventive Dentistry,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院口腔预防科,郑州450052

出　　处：《华西口腔医学杂志》2023年第5期533-540,共8页West China Journal of Stomatology

摘　　要：目的探讨慢性牙周炎(CP)和慢性阻塞性肺疾病(COPD)之间潜在的cross-talk基因、相关通路和转录因子。方法从GEO数据库下载CP(GSE10334和GSE16134)和COPD(GSE76925)的基因表达谱,进行差异表达分析、功能聚类分析、构建蛋白质相互作用(PPI)网络,通过4种拓扑分析算法和模块划分筛选出核心cross-talk基因,再进行功能聚类分析。结果GSE10334共筛选出164个差异表达基因(DEG)(119个上调、45个下调);GSE16134共筛选出208个DEG(154个上调、54个下调);GSE76925共筛选出1408个DEG(557个上调、851个下调)。PPI网络包括21个节点和20条边。最终筛选出7个核心基因CD79A、FCRLA、CD19、IRF4、CD27、SELL和CXCL13,相关通路为原发性免疫缺陷,B细胞受体信号通路和细胞因子-细胞因子受体相互作用。其得分最高的转录因子为IRF4。结论本研究探索了CP和COPD的潜在共同发病机制,其cross-talk基因、相关通路和转录因子可能为进一步的机制研究提供新思路。Objective To investigate possible cross-talk genes,associated pathways,and transcription factors between chronic periodontitis(CP)and chronic obstructive pulmonary disease(COPD).Methods The gene expression profiles of CP(GSE10334 and GSE16134)and COPD(GSE76925)were downloaded from the GEO database.Differential expression and functional clustering analyses were performed.The protein‑protein interaction(PPI)network was constructed.The core cross-talk genes were filtered using four topological analysis algorithms and modular segmentation.Then,functional clustering analysis was performed again.Results GSE10334 detected 164 differentially expressed genes(DEGs)(119 upregulated and 45 downregulated).GSE16134 identified 208 DEGs(154 upregulated and 54 downregulated).GSE76925 identified 1408 DEGs(557 upregulated and 851 downregulated).The PPI network included 21 nodes and 20 edges.The final screening included seven cross-talk genes:CD79A,FCRLA,CD19,IRF4,CD27,SELL,and CXCL13.Relevant pathways included primary immunodeficiency,the B-cell receptor signaling pathway,and cytokine-cytokine receptor interaction.Conclusion This study indicates the probability of shared pathophysiology between CP and COPD,and their cross-talk genes,associated pathways,and transcription factors may offer novel concepts for future mechanistic investigations.

关 键 词：慢性牙周炎 慢性阻塞性肺疾病 cross-talk基因 转录因子 

分 类 号：R781.42[医药卫生—口腔医学]