鄱阳湖湿地不同环境条件土壤AM真菌群落特征及其影响因素  

Characteristics and Influencing Factors of Soil Arbuscular Mycorrhizal Fungi Community Under Different Environmental Conditions in Poyang Lake Wetland

作　　者：王金平 袁继红 任琼[2] 周莉荫[2] 缪泸君 万方 徐盛丁 万松贤 WANG Jinping;YUAN Jihong;REN Qiong;ZHOU Liyin;MIAO Lujun;WAN Fang;XU Shengding;WAN Songxian(Nanchang Institute of Technology,Nanchang 330099;Jiangxi Academy of Forestry,Nanchang 330032;Nanchang Urban Planning and Design Institute,Nanchang 330013;Nanji Wetland National Nature Reserve Agency,Nanchang 330038)

机构地区：[1]南昌工程学院,南昌330099 [2]江西省林业科学院,南昌330032 [3]南昌市城市规划设计研究总院,南昌330013 [4]江西鄱阳湖南矶湿地国家级自然保护区管理局,南昌330038

出　　处：《水土保持学报》2023年第5期252-258,共7页Journal of Soil and Water Conservation

基　　金：江西省林业科学院博士后项目(2020522302);江西省林业科学院博士启动项目(2020522301);2021年中央财政湿地保护与恢复补助重要湿地生态状况监测评价项目(赣林函字〔2022〕79号);江西省林业局林业科技创新专项([2021]06号);江西省林业科学院基础研究与人才科研专项(2022511601)。

摘　　要：丛枝菌根(AM)真菌可以与湿地植物共生,并在湿地生态系统的修复与维护过程中具有重要作用。然而,不同环境条件下湿地土壤AM真菌群落分布特征及其影响因素仍然有待明晰。选择鄱阳湖湿地不同水位条件(高水位和低水位)2种典型植物群落(南荻和水蓼)为研究对象,运用高通量测序技术探究水位和植被类型对鄱阳湖湿地土壤AM真菌群落结构和多样性的影响,比较不同环境条件下AM真菌群落结构和多样性差异,分析其与土壤理化性质的关系。结果表明,AM真菌OTU数为21~38,水位和植被类型影响鄱阳湖湿地土壤AM真菌OTU数,高水位下AM真菌OTU数高于低水位,水蓼群落高于南荻群落。被鉴别出的菌属为Glomus、Claroideoglomus和Paraglomus,其中,Glomus是优势属,占比85%以上,其相对丰度在高水位下显著高于低水位(p<0.05),南荻群落高于水蓼群落。AM真菌多样性指数(Simpson和Shannon指数)受水位和植被类型以及二者交互影响(p<0.01),低水位下不同群落之间的AM真菌多样性指数无显著差异,而高水位下水蓼群落AM真菌多样性指数显著高于南荻群落(p<0.05)。水位对AM真菌群落结构的影响高于植被类型,只有在低水位下植被类型才显著影响AM真菌群落结构。相关性分析显示,AM真菌多样性指数(Shannon和Simpson指数)与土壤因子无显著关系,低水位下土壤含水量与Glomus和OTU数量呈显著负相关(p<0.05),而高水位下pH与优势属Glomus数量呈显著正相关(p<0.05)。因此,湿地生态系统中土壤含水量对Glomus的影响存在阈值效应。研究结果能为退化湿地生态系统修复提供科学指导。Arbuscular mycorrhizal(AM)fungi can coexist with wetland plants and play an important role in the restoration and maintenance of wetland ecosystem.However,the distribution and influencing factors of soil AM fungal community under different environmental conditions in wetland ecosystem still need to be clarified.Therefore,this study used high-throughput sequencing technology to explore the effects of water level and vegetation type on the community structure and diversity of soil AM fungi in Poyang Lake wetland.Two typical plant communities(Triarrhena lutarioriparia and Persicaria hydropiper)under different water levels(high and low water level)were chosen to compare the difference of the community structure and diversity of AM fungi among different environmental conditions.The relationship between AM fungal community and soil physicochemical properties was analyzed.The results showed that the OTU number of AM fungi ranged from 21 to 38.Both water level and vegetation type affected the OTU number of AM fungi in Poyang Lake wetland.The OTU number of AM fungi was higher under high water level than that under low water level,and it was higher in P.hydropiper community than that in T.lutarioriparia community.The identified genera of AM fungi were Glomus,Claroidoglomus and Paraglomus.Among them,Glomus was the dominant genus,accounting for more than 85%.And the relative abundance of Glomus was significantly higher under high water level,compared with low water level,while it was significantly higher in T.lutarioriparia community,compared with P.hydropiper community(p<0.05).The AM fungal diversity indices(Simpson and Shannon index)were affected by water level,vegetation type and their interaction(p<0.01).Under low water level,there was no significant difference in AM fungal diversity indices between different communities,but under high water level,the AM fungal diversity indices in P.hydropiper community was significantly higher than that in T.lutarioriparia community(p<0.05).Water level had a higher effect on community

关 键 词：湿地 丛枝菌根真菌群落 水位 植被类型 土壤环境因子 

分 类 号：X172[环境科学与工程—环境科学]