外源褪黑素对镉胁迫烟草幼苗的缓解效应  

Alleviative Effect of Exogenous Melatonin on Cadmium Stress in Tobacco Seedlings

作　　者：吕怡颖 郑元仙 王继明 胡骞予 蔡宪杰[3] 陈小龙 余磊[1] 黄飞燕 韩天华 LÜYiying;ZHENG Yuanxian;WANG Jiming;HU qianyu;CAI Xianjie;CHEN Xiaolong;YU Lei;HUANG Feiyan;HAN Tianhua(Kunming University,Yunnan Engineering and Technology Research Center of Urban Characteristic Agriculture,Kunming 650214,China;Lincang Branch of Yunnan Tobacco Company,Lincang,Yunnan 677000,China;Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China;China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450000,China;Yunnan Tobacco Company Lijiang,Lijiang,Yunnan 674100,China)

机构地区：[1]昆明学院,云南省都市特色农业工程技术研究中心,昆明650214 [2]云南省烟草公司临沧市公司,云南临沧677000 [3]上海烟草集团有限责任公司,上海200082 [4]河南中烟工业有限责任公司,郑州450000 [5]云南省烟草公司丽江市公司,云南丽江674100

出　　处：《中国烟草科学》2023年第4期25-32,共8页Chinese Tobacco Science

基　　金：上海烟草集团有限责任公司基地项目(2022310000140544);云南省烟草公司临沧市公司科技计划一般项目(2023530900242001、2022530900242001、2021530000242023);云南省烟草公司丽江市公司科技计划一般项目(2022530700242001)。

摘　　要：为探究镉胁迫对烟草生长的影响及外源褪黑素对烟草镉毒害的缓解作用,本试验以云烟100为材料,通过水培试验研究不同镉浓度对烟草幼苗农艺性状、SPAD值、叶片Cd^(2+)含量的影响以及不同浓度外源褪黑素(MT)(10、50、100、200μmol/L)预处理对100μmol/LCdCl2胁迫下烟草毒害症状的缓解效应。结果显示,镉浓度50~200μmol/L对烟苗生长发育具有显著抑制作用,外源MT可显著缓解烟草镉毒害。与对照相比,200μmol/L褪黑素处理的云烟100根长、鲜质量、最大叶长、最大叶宽、株高及叶SPAD值分别增加了17.50%、131.89%、70.83%、51.79%、78.43%和87.20%;MDA和叶片镉含量分别降低了42.67%和57.4%;超氧化物歧化酶(SOD)、过氧化物酶(POD)、谷胱甘肽过氧化物酶(GSH)含量、可溶性蛋白(SP)含量分别增加了391.20%、3305.57%、332.11%和88.52%。综上,外源褪黑素可通过减少叶片镉富集,提高抗氧化酶活性和细胞渗透压调控物质来提高烟株自身抗逆性,从而缓解烟草Cd毒害。In order to explore the effect of cadmium stress on tobacco growth and the mitigation effect of exogenous melatonin(MT)on cadmium toxicity,the effects of different concentrations of cadmium on agronomic traits,SPAD values and cadmium content in leaves of Yunyan 100 seedlings were studied by hydroponic experiments,and the alleviating effects of different concentrations of exogenous MT(10,50,100,200μmol/L)pretreatments on tobacco toxicity under 100μmol/L CdCl2 stress in tobacco were studied.The results showed that cadmium(50-200μmol/L)could significantly inhibit the growth and development of tobacco seedlings,and exogenous MT could significantly alleviate the toxicity of cadmium in tobacco.Compared with the cadmium(100μmol/L)treatment alone,the root length,fresh mass,maximum leaf length,maximum leaf width,plant height and SPAD values under the 200μmol/L melatonin treatment increased by 17.50%,131.89%,70.83%,51.79%,78.43%and 87.20%,respectively.MDA and intracellular cadmium content decreased by 42.67%and 57.4%,respectively.The activities of superoxide dismutase(SOD),peroxidase(POD),glutathione peroxidase(GSH)and the content of soluble protein(SP)increased by 391.20%,3305.57%,332.11%and 88.52%,respectively.In conclusion,exogenous melatonin can reduce cadmium enrichment in leaves,improve antioxidant enzyme activities and cytosmotic pressure regulators of tobacco seedlings,and improve the stress resistance of tobacco plants,so as to alleviate Cd toxicity in tobacco.

关 键 词：镉胁迫 烟草 褪黑素 抗氧化能力 

分 类 号：S572.01[农业科学—烟草工业]