生防菌BZ3对烟草普通花叶病毒的生防效果及全基因组分析  

Biocontrol Effects of Antagonistic Strain BZ3 on Tobacco Mosaic Virus and the Antagonistic Strain Genome Analysis

作　　者：刘蕾 肖志鹏 周向平 肖艳松 蔡海林 李玲玲 梅斯钇 刘天波 唐前君[1] LIU Lei;XIAO Zhipeng;ZHOU Xiangping;XIAO Yansong;CAI Hailin;LI Lingling;MEI Siyi;LIU Tianbo;TANG Qianjun(College of Plant Protection,Hunan Agricultural University,Changsha 410128,China;Hunan Provincial Tobacco Compony,Changsha 410004,China)

机构地区：[1]湖南农业大学植物保护学院,长沙410128 [2]中国烟草总公司湖南省公司,长沙410004

出　　处：《中国烟草科学》2023年第4期33-40,共8页Chinese Tobacco Science

基　　金：湖南省重点领域研发计划项目(2023NK2019);湖南省烟草公司衡阳市公司项目(HYYC2023KJ32)。

摘　　要：利用稀释分离法从烟草根际土壤中分离筛选获得抗烟草普通花叶病毒(Tobaccomosaicvirus,TMV)的优良生防菌株BZ3,通过形态、生理生化和分子生物学鉴定菌株种类,评价其对TMV的生防效果,利用全基因组测序及生物信息学分析其次生代谢产物合成相关基因簇。结果表明,从根际土壤中分离的BZ3菌株鉴定为贝莱斯芽孢杆菌(Bacillusvelezensis),其发酵液对TMV的盆栽和田间小区防效分别为58.97%和72.07%;基因功能预测显示菌株BZ3基因组中含有表面活性素等多种具有抑菌活性的次生代谢产物合成相关基因簇。本研究表明,BZ3菌株对TMV生防效果优良,抑菌谱较广,基因组中含有大量抑菌活性物质合成相关的基因簇,具有作为TMV生防菌剂的潜力。An excellent biocontrol strain BZ3 against tobacco mosaic virus(TMV)was isolated and screened from tobacco rhizosphere soil by the dilution separation method.The strain was identified by morphological,physiological and biochemical,as well as molecular biology analyses,and its biocontrol effects on TMV were evaluated.In addition,the strain was further analyzed by whole genome sequencing and bioinformatics.The results showed that the BZ3 strain was identified as Bacillus velezensis,and the control effects of its fermentation broth on TMV in pot and field plots were 58.97%and 72.07%,respectively.Gene function prediction showed that the genome of strain BZ3 contained many gene clusters related to the synthesis of secondary metabolites with antibacterial activities,such as surfactin.This study suggested that the BZ3 strain has excellent biocontrol effects on TMV,and the antibacterial spectrum is wide.Its genome contains a large number of gene clusters related to the synthesis of antibacterial active substances,exhibiting the potential as a biocontrol agent against TMV.

关 键 词：烟草普通花叶病毒 生物防治 贝莱斯芽孢杆菌 全基因组测序 次生代谢产物 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]