冻害胁迫下马铃薯叶片中生物碱类物质研究  

Study on Alkaloids in Potato Leaves under Freezing Stress

作　　者：刘香 暴会会 张镇杰 杨福丽 罗永超 祝光涛 LIU Xiang;BAO Huihui;ZHANG Zhengjie;YANG Fuli;LUO Yongchao;ZHU Guangtao(Yunnan Provincial Key Laboratory of Potato Biology,Potato Science Research Institute,School of Life Science,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学生命科学学院,马铃薯科学研究院,云南省马铃薯生物学重点试验室,云南昆明650500

出　　处：《云南师范大学学报（自然科学版）》2023年第5期19-24,共6页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家重点研发计划资助项目(2022YFF1002500).

摘　　要：以耐寒性野生马铃薯材料Solanum commersonii和冷冻敏感材料StGp Phureja作为研究对象,4℃驯化3 d后-3℃冻害处理3 h、6 h、12 h进行转录组和代谢组测序及相关分析.研究表明,生物碱类物质是这两个马铃薯品种有差异的主要代谢产物类别之一,对其抗寒性有重要影响.在马铃薯耐寒材料中有12个差异生物碱类代谢物响应低温胁迫.根据这12个核心差异代谢物进行关联分析筛选出27个转录因子与之有相似的表达模式,这些转录因子可能参与生物碱的调控.In this study,the cold-tolerant wild potato variety S.commersonii and the freeze-sensitive species StGp Phureja were used as materials.The transcriptome and metabolome sequencing and correlation analysis were conducted after acclimation at 4℃for 3 d and freezing treatment at-3℃for 3 h,6 h and 12 h.The results showed that alkaloids were one of the main metabolites that led to the difference of low temperature tolerance between the two potato varieties,and had an important effect on their cold resistance.There are 12 different alkaloid metabolites in the cold resistant materials that play the role of cold resistance.According to the 12 core differential metabolites,27 transcription factors were selected by association analysis with similar expression patterns,which may be involved in the regulation of alkaloids.

关 键 词：马铃薯 转录组 代谢组 生物碱 低温 

分 类 号：S532[农业科学—作物学]