马铃薯Solanum commersonii叶片再生体系建立  

Establishment of Regeneration System Leaf of Solanum Commersonii

作　　者：暴会会 周钰洁 罗永超 刘希 付婉婷 祝光涛 BAO Huihui;ZHOU Yujie;LUO Yongchao;LIU Xi;FU Wanting;ZHU Guangtao(Yunnan Provincial Key Laboratory of Potato Biology,Potato Science Research Institute,School of Life Science,Yunnan Normal University,Kunming 650500,China)

机构地区：[1]云南师范大学生命科学学院,马铃薯科学研究院,云南省马铃薯生物学重点试验室,云南昆明650500

出　　处：《云南师范大学学报（自然科学版）》2023年第5期25-28,共4页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家重点研发计划资助项目(2022YFF1002500).

摘　　要：以马铃薯野生材料Solanum commersonii叶片为研究对象,经过对10个不同激素组合的筛选,得到最佳配比,建立马铃薯野生材料叶片再生体系.结果表明,马铃薯野生材料S.commersonii叶片愈伤的最佳诱导培养基为MS+1.00 mg·L^(-1)吲哚乙酸+1.50 mg·L^(-1)玉米素,诱导率高达100%,生长情况较好,愈伤组织呈浅绿色,表面呈米粒状.叶片愈伤组织不定芽分化的最佳培养基为MS+1.00 mg·L^(-1)吲哚乙酸+2.00 mg·L^(-1)玉米素,不定芽分化率达66.67%.Using S.commersonii leaves of potato wild materials as experimental material,a medium with the optimal ratio of hormones was obtained through screening of ten different hormone compositions,and we established the leaf regeneration system of potato wild materials.The results showed that MS+1.00 mg·L^(-1) IAA+1.50 mg·L^(-1) ZT was the best medium for inducing callus of the wild potato material S.commersonii.The induction rate was up to 100 percent and the growth condition was good.The callus cells were light green and the surface was rice granular.The best medium for adventitious bud differentiation of callus on leaves was MS+1.00 mg·L^(-1) IAA+2.00 mg·L^(-1) ZT,and the adventitious bud differentiation rate was 66.67 percent.

关 键 词：马铃薯 S.commersonii 愈伤组织 不定芽分化 再生体系 

分 类 号：S532[农业科学—作物学]