白藜芦醇对脱矿牙本质仿生再矿化及粘接耐久性的分析  

Effect of resveratrol on biomimetic remineralization of demineralized dentin and resin-dentin bond durability

作　　者：孙丽君 何峰[2] SUN Lijun;HE Feng(Department of Stomatology,Jiangsu Province Hospital of Traditional Chinese Medicine,Nanjing 210029,China)

机构地区：[1]江苏省中医院口腔科,江苏南京210029 [2]南京医科大学附属口腔医院口腔特诊科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,江苏南京210029

出　　处：《口腔医学》2023年第9期781-785,共5页Stomatology

基　　金：江苏省科教能力提升工程——江苏省研究型医院(YJXYYJSDW4);江苏省医学创新中心(CXZX202227)。

摘　　要：目的 评价白藜芦醇对脱矿牙本质仿生再矿化及树脂-牙本质粘接耐久性的影响。方法 低速切割机下切取的中层牙本质经35%磷酸酸蚀脱矿后随机分为3组并分别给予蒸馏水浸泡(Ctr组)、直接再矿化处理7 d(Pos组)和白藜芦醇预处理30 min后再矿化处理7 d(Res组)。采用扫描电镜观察牙本质表面矿物质生成情况,X射线衍射和衰减全反射傅里叶变换红外光谱分析牙本质表面晶体的特征,微拉伸强度实验观察树脂-牙本质粘接耐久性。结果 扫描电镜发现仿生再矿化处理的各组牙本质表面均可以形成矿物晶体,白藜芦醇预处理后的牙本质表面更为明显,新形成的晶体经X射线衍射和衰减全反射傅里叶变换红外光谱分析为羟基磷灰石。与没有预处理剂的阳性对照组相比,白藜芦醇预处理显著增加了初始微拉伸强度值。微拉伸强度值在老化过程中减小,但白藜芦醇预处理的样本该值下降程度最低。结论 白藜芦醇预处理可以促进脱矿牙本质的仿生再矿化并改善树脂-牙本质的粘接耐久性。Objective To evaluate the contributions of resveratrol to dentin biomimetic remineralization and resin-dentin bond durability.Methods The middle dentin slices cut by a low-speed diamond saw were etched with 35% phosphoric acid and divided into three groups,which were respectively immersed in distilled water(Ctr group),directly remineralized for 7 d(Pos group),and pretreated with resveratrol for 30 min and then remineralized for 7 d(Res group).The formation of minerals on the dentin surface was observed by scanning electron microscope(SEM),and the crystal characteristics were analyzed by X-ray diffraction(XRD)and attenuated total reflection Fourier transform infrared spectroscopy(ATR-FTIR).Micro tensile strength was used to observe the resin-dentin bond durability.Results SEM showed that the dentin after remineralization could form crystals,and more crystals were formed on the resveratrol-pretreated dentin surface.XRD and ATR-FTIR analysis showed that the newly formed crystal was hydroxyapatite(HA).Pretreatment with resveratrol significantly increased the initial micro-tensile strength compared with the positive control group without primer conditioning.Micro-tensile strength decreased during aging,however,the decrease in resveratrol-pretreated samples was the least significant.Conclusion Resveratrol can promote the biomimetic remineralization of demineralized dentin and improve resin-dentin bond durability.

关 键 词：白藜芦醇 牙本质 仿生再矿化 粘接耐久性 

分 类 号：R783.1[医药卫生—口腔医学]