马铃薯种质资源抗病毒分子标记辅助筛选  

Molecular Marker-Assisted Screening of Potato Germplasm Resources for Virus Resistance

作　　者：娄树宝[1,2] 杨梦平 邢金月[1] 翟玲侠 王辉 刘春生[1] 王立春[1] 宋继玲[1] Lou Shubao;Yang Mengping;Xing Jinyue;Zhai Lingxia;Wang Hui;Liu Chunsheng;Wang Lichun;Song Jiling(Keshan Branch of Heilongjiang Academy of Agricultural Sciences/Test-Tube Plantlet Bank of National Potato Germplasm Resources(Keshan),Qiqihar 161005,Heilongjiang,China;Academy of Agricultural and Forestry Sciences,Qinghai University,Xining 810016,Qinghai,China)

机构地区：[1]黑龙江省农业科学院克山分院/国家马铃薯种质资源试管苗库(克山),黑龙江齐齐哈尔161005 [2]青海大学农林科学院,青海西宁810016

出　　处：《作物杂志》2023年第4期65-70,共6页Crops

基　　金：齐齐哈尔市科技计划创新激励项目(CNYGG-2022025);科技部、财政部、国家科技资源共享服务平台项目“国家作物种质资源库马铃薯分库运行服务”(NCGRC-2022-44);农业农村部物种保护项目“马铃薯种质资源安全保存及普查收集资源鉴定评价与繁殖编目入库”(19221860);农业农村部“马铃薯种质资源精准鉴定”(19221974)。

摘　　要：马铃薯Y病毒(PVY)和马铃薯X病毒(PVX)是对马铃薯产量和品质影响最主要的2种病毒,Ry_(adg)、Ry_(sto)和Ry_(chc)是对PVY有极端抗性的基因,Rx是对PVX有极端抗性的重要基因。利用与抗性基因Ry_(adg)、Ry_(sto)、Ry_(chc)和Rx紧密连锁的分子标记,对国内外102个马铃薯品种进行了标记检测。结果表明,含有YES3-3B标记的材料最多,占供试材料99.02%;含有Rxsp标记的材料最少,占10.78%;只含有1个标记的品种有4个,占3.92%;同时含有2个标记的品种有62个,占60.78%;同时含有3个标记的品种有30个,占29.41%;同时含有4个标记的品种有6个,分别是陇薯11号、S.acaule、富金、云薯105、晋薯8号和大西洋,占供试材料的5.88%。Potato Y virus(PVY)and potato X virus(PVX)are the two most important viruses that affect the yield and quality of potato,Ry_(adg),Ry_(sto)and Ry_(chc)are important genes with extreme resistance to PVY,Rx is an important gene with extreme resistance to PVX.Four molecular markers closely related to the resistance genes Ry_(adg),Ry_(sto),Ry_(chc)and Rx were used to detected 102 potato varieties.The results showed that the most materials contained YES3-3B markers,accounting for 99.02%of the test materials;the least materials contained Rxsp markers,accounting for 10.78%of the test materials;four varieties contained only one marker,accounting for 3.92%of the test materials;the 62 varieties contained two markers,accounting for 60.78%of the test materials;and the 30 varieties contained three markers,accounting for 29.41%.There were six varieties containing four markers,which were‘Longshu 11’,‘S.acaule’,‘Fujin’,‘Yunshu 105’,‘Jinshu 8’and‘Atlantic’,accounting for 5.88%of the test material.

关 键 词：马铃薯 病毒 分子标记 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]