Uncovering pharmacological mechanisms of Phellinus linteus on focal segmental glomeruloscleosis rats through tandem mass tag-based quantitative proteomic analysis,network pharmacology analysis and experimental validation  

作　　者：WAN Feng YANG Ruchun TANG Yuewen 

机构地区：[1]Department of Nephrology,Key Laboratory of Kidney Disease Prevention and Control Technology,Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University,Hangzhou 310007,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第4期744-750,共7页中医杂志（英文版）

基　　金：Public Welfare Technology Application Research Program of Zhejiang Province:Study on the Application of Phellinus linteus Protocatechualdehyde in Inhibiting Glomerular Extracellular Matrix Accumulation based on TSP-1 Double Luciferase Reporting System(No.LGC21H290002);Key Projects of Zhejiang Administration of Traditional Chinese Medicine:Quality and Safety Evaluation of Cultivated Phellinus linteus in Zhejiang Province and its Comprehensive Application in the Prevention and Treatment of Kidney Disease(No.2020ZZ016)。

摘　　要：OBJECTIVE:To explore the underlying molecular mechanism of Phellinus linteus(P.linteus).METHODS:We used a tandem mass tag-based quantitative proteomic method to determine the differentially expressed proteins.Network pharmacology analysis was used to analysis the main components of P.linteus and construct the compound-target network.Western blotting and quantitative real-time polymerase chain reaction(qRT-PCR)were used to validate the analyses results.RESULTS:The expression levels of thrombospondin-1(TSP-1)and transforming growth factor(TGF)-β1/Smad3 signaling pathway proteins were significantly upregulated in focal segmental glomeruloscleosis(FSGS)rats.The P.linteus reduced the expression levels of TSP-1 and TGF-β1 signaling pathway proteins.Network pharmacology analysis revealed that protocatechualdehyde was the main active component.Subsequent in vivo and in vitro experiments validated the results of proteomic and network pharmacology analyses.CONCLUSIONS:Our results suggested that P.linteus may inhibit renal sclerosis by inhibiting TSP-1-activated TGF-β1 signaling and may have potential applications in the treatment of FSGS.

关 键 词：PHELLINUS GLOMERULOSCLEROSIS focal segmental thrombospondin 1 transforming growth factor beta1 smad3 protein 

分 类 号：R285[医药卫生—中药学]