Efficacy of electroacupuncture stimulating Zusanli(ST36)and Xuanzhong(GB39)on synovial angiogenesis in rats with adjuvant arthritis  

作　　者：JIANG Jianzhen ZHANG Xin LUO Zhenguo SU Chengguo ZHOU Haiyan JIANG Yuqing XIAO Xianjun CHEN Yunfei ZHU Jun 

机构地区：[1]School of Acupuncture-Moxibustion and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [2]Vocational Training Department,Acupuncture and Moxibustion School Affiliated to Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China [3]Department of Acupuncture,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China

出　　处：《Journal of Traditional Chinese Medicine》2023年第5期955-962,共8页中医杂志（英文版）

基　　金：Natural Science Foundation-funded Project:Study of Electroacupuncture on the Angiogenesis in Rheumatoid Arthritis Rats by HIF-1α/MDM2/p53 Signaling Pathway(No.81704152);Study on Immune Mechanisms of Macrophage M1/M2 Polarization in the Treatment of Rheumatoid Arthritis by Moxibustion“trengthening Body Resistance and Eliminating Evil”(No.81973959);Foundation of Science and Technology Department of Sichuan Province:Study of Electroacupuncture on the Punnus in Rheumatoid Arthritis Rats by HIF-1α,STAT3,Noth1 Signaling Pathway(No.2019YJ0491)。

摘　　要：OBJECTIVE:To investigate the efficacy of electroacupuncture(EA)stimulating Zusanli(ST36)and Xuanzhong(GB39)on synovial angiogenesis in rats with adjuvant arthritis(AA).METHODS:AA models were established by bilateral injection of Freund's complete adjuvant(FCA)in male Sprague-Dawley rats.Three days after injection,rats were given EA at Zusanli(ST36)and Xuanzhong(GB39)acupoints,once every other day,for 16 d.The arthritis index score,paw volume,and hematoxylin-eosin(HE)staining was performed for each animal.Angiogenesis marker cluster of differentiation 34(CD34)expression and synovial cell apoptosis in synovial tissue were observed.The levels of Notch1,hairy and enhancer of split homolog-1(Hes1),transforming growth factor-beta(TGF-β)and basic fibroblast growth factor(bFGF)were subsequently detected.RESULTS:We found that EA significantly decreased arthritis index scores,paw volume,and HE staining scores.EA could significantly inhibit the expression of CD34,promoting apoptosis of synovial cells in the joint synovial tissue of AA rats.The expression of Notch1 signaling pathway proteins and mR NAs(Notch1,Hes1,TGF-β,and bFGF)were markedly downregulated by EA treatment.CONCLUSIONS:These results prove that EA attenuates synovial angiogenesis by inhibiting the Notch1 signaling pathway in AA rat models.Based on our findings,we propose that EA is a promising complementary and alternative therapy in rheumatoid arthritis.

关 键 词：ELECTROACUPUNCTURE ARTHRITIS experimental synovial angiogenesis receptor Notch1 signal transduction 

分 类 号：R245.97[医药卫生—针灸推拿学]